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Literature summary for 1.1.1.175 extracted from

  • Yamanaka, K.; Gino, M.; Kaneda, R.
    A specific NAD-D-xylose dehydrogenase from Arthrobacter sp. (1977), Agric. Biol. Chem., 41, 1493-1499.
No PubMed abstract available
Search for more literature for 1.1.1.175

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
17.4
-
 D-xylose
-
 Arthrobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
 gel filtration Arthrobacter sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-xylose + NAD+ Arthrobacter sp. highly specific for both substrates D-xylonolactone + NADH + H+
-
 ?
D-xylose + NAD+ Arthrobacter sp. 588 highly specific for both substrates D-xylonolactone + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Arthrobacter sp.
-
-
-
Arthrobacter sp. 588
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography techniques Arthrobacter sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:D-xylose-grown cell inducible enzyme synthesis by growth in D-xylose-containing medium Arthrobacter sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
24.12
-
-
 Arthrobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-xylose + NAD+ highly specific for both substrates Arthrobacter sp. D-xylonolactone + NADH + H+
-
 ?
D-xylose + NAD+ highly specific for both substrates Arthrobacter sp. 588 D-xylonolactone + NADH + H+
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 35
-
 Arthrobacter sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 stable below Arthrobacter sp.
50
-
 complete inactivation Arthrobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10.4
-
-
 Arthrobacter sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 7.8 stable within, no enzymatic activity Arthrobacter sp.

Cofactor

Cofactor Comment Organism Structure
NAD+ cannot be replaced by NADP+ Arthrobacter sp.