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Literature summary for 1.1.1.170 extracted from

  • Rahimtula, A.D.; Gaylor, J.L.
    Investigation of the component reactions of oxidative sterol demethylation. Partial purification of a microsomal sterol 4alpha-carboxylic acid decarboxylase (1972), J. Biol. Chem., 247, 9-15.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
5alpha-cholest-7-en-3-one 2-9.3% inhibition between 16 and 130 nmol Rattus sp.
5alpha-Cholest-7-en-3beta-ol 15.5-65% inhibition between 30 and 260 nmol Rattus sp.
Fe2+ less than 25% inhibition at 1 mM Rattus sp.
Zn2+ marked inhibition between 0.1 and 1 mM Rattus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-en-4alpha-oic acid
-
Rattus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3beta-hydroxy-4alpha-methyl-5alpha-cholest-7-ene-4beta-carboxylate + NAD+ Rattus sp.
-
4alpha-methyl-5alpha-cholest-7-en-3-one + CO2 + NADH
-
?
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-en-4alpha-oic acid + NAD+ Rattus sp. no epimerization of 4beta-isomer into 4alpha-isomer 4alpha-methyl-5alpha-cholest-7-en-3-one + CO2 + NADH
-
ir

Organism

Organism UniProt Comment Textmining
Rattus sp.
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phospholipoprotein presence of phosphatidylcholine and phosphatidylethanolamine, no loss of activity after removal of phospholipids Rattus sp.

Purification (Commentary)

Purification (Comment) Organism
near homogeneity, chromatography techniques, sodium deoxycholate solubilization Rattus sp.

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.11
-
-
Rattus sp.

Storage Stability

Storage Stability Organism
4°C, 10 mM phosphate buffer, pH 7.4, no loss of activity within 2 weeks Rattus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3beta-hydroxy-4alpha-methyl-5alpha-cholest-7-ene-4beta-carboxylate + NAD+
-
Rattus sp. 4alpha-methyl-5alpha-cholest-7-en-3-one + CO2 + NADH
-
?
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-en-4alpha-oic acid + NAD+ no epimerization of 4beta-isomer into 4alpha-isomer Rattus sp. 4alpha-methyl-5alpha-cholest-7-en-3-one + CO2 + NADH
-
ir

Synonyms

Synonyms Comment Organism
4alpha-carboxylic acid decarboxylase
-
Rattus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9
-
Rattus sp.

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
acidic pH results in a very low decarboxylation rate Rattus sp.

Cofactor

Cofactor Comment Organism Structure
NAD+ can not be replaced by NADP+ Rattus sp.