Application | Comment | Organism |
---|---|---|
synthesis | hydrogen transfer from formate to D-fructose 6-phosphate, mediated by NAD(H) and catalyzed by a coupled enzyme system of purified Candida boidinii formate dehydrogenase and AfM1PDH, is used for the preparative synthesis of D-mannitol 1-phosphate or, by applying an analogous procedure using deuterio formate, the 5-[2H] derivative thereof, overview | Aspergillus fumigatus |
Cloned (Comment) | Organism |
---|---|
high level overexpression in Escherichia coli strain JM109 | Aspergillus fumigatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic isotope effects, overview | Aspergillus fumigatus | |
0.016 | - |
NADH | pH 7.1, 25°C, recombinant enzyme | Aspergillus fumigatus | |
0.23 | - |
D-mannitol 1-phosphate | pH 7.1, 25°C, recombinant enzyme | Aspergillus fumigatus | |
0.75 | - |
NAD+ | pH 7.1, 25°C, recombinant enzyme | Aspergillus fumigatus | |
2.1 | - |
D-fructose 6-phosphate | pH 7.1, 25°C, recombinant enzyme | Aspergillus fumigatus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, recombinant enzyme, SDS-PAGE | Aspergillus fumigatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-mannitol 1-phosphate + NAD+ | Aspergillus fumigatus | - |
D-fructose 6-phosphate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus fumigatus | Q4X1A4 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme 2.1fold from Escherichia coli by affinity chromatography | Aspergillus fumigatus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
D-mannitol 1-phosphate + NAD+ = D-fructose 6-phosphate + NADH + H+ | the hydride transfer is strongly rate-determining for the overall enzymatic reaction | Aspergillus fumigatus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
17.6 | - |
oxidation reaction, purified recombinant enzyme | Aspergillus fumigatus |
169 | - |
reduction reaction, purified recombinant enzyme | Aspergillus fumigatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-mannitol 1-phosphate + NAD+ | - |
Aspergillus fumigatus | D-fructose 6-phosphate + NADH + H+ | - |
r | |
D-mannitol 1-phosphate + NAD+ | M1PDH is stereospecific for transferring the hydrogen to NAD+, in situ proton NMR studies of enzymatic oxidation of D-5-[2H]-mannitol 1-phosphate, overview | Aspergillus fumigatus | D-fructose 6-phosphate + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, recombinant enzyme, SDS-PAGE | Aspergillus fumigatus |
Synonyms | Comment | Organism |
---|---|---|
long-chain mannitol-1-phosphate 5-dehydrogenase | - |
Aspergillus fumigatus |
M1PDH | - |
Aspergillus fumigatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Aspergillus fumigatus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.5 | - |
D-mannitol 1-phosphate | pH 7.1, 25°C, recombinant enzyme | Aspergillus fumigatus | |
125 | - |
D-fructose 6-phosphate | pH 7.1, 25°C, recombinant enzyme | Aspergillus fumigatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.1 | - |
- |
Aspergillus fumigatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | poor activity with NADP(H) | Aspergillus fumigatus | |
NAD+ | dependent on | Aspergillus fumigatus | |
NADH | dependent on | Aspergillus fumigatus |