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Literature summary for 1.1.1.17 extracted from
- D-Mannitol-1-phosphate dehydrogenase from E. coli (1955), Methods Enzymol., 1, 346-348.
No PubMed abstract available
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| intracellular | - |
Escherichia coli | 5622 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-mannitol 1-phosphate + NAD+ | Escherichia coli | highly specific for both substrates | D-fructose 6-phosphate + NADH + H+ | - |
r |  |
| D-mannitol 1-phosphate + NAD+ | Escherichia coli | favours reverse reaction | D-fructose 6-phosphate + NADH + H+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| precipitation techniques | Escherichia coli |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Escherichia coli | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
1 unit defined as change of 0.01 in optical density at 340 nm during 30-90 second interval | Escherichia coli |
| additional information | - |
860 U/mg protein | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-mannitol 1-phosphate + NAD+ | highly specific for both substrates | Escherichia coli | D-fructose 6-phosphate + NADH + H+ | - |
r | |
| D-mannitol 1-phosphate + NAD+ | favours reverse reaction | Escherichia coli | D-fructose 6-phosphate + NADH + H+ | - |
r | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
45% loss of activity in 10 min | Escherichia coli |
| 60 | - |
complete inactivation in 10 min | Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | no sharp optimum | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | strictly specific | Escherichia coli | |
| NADH | strictly specific | Escherichia coli | |
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Release 2023.1 (January 2023)
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