Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | competitive inhibition | Brochothrix thermosphacta | |
ATP | competitive inhibition | Brochothrix thermosphacta |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
NADH | higher affinity for NADH | Brochothrix thermosphacta | |
0.05 | - |
NAD+ | - |
Brochothrix thermosphacta | |
0.05 | - |
D-mannitol 1-phosphate | cosubstrate: NAD+ | Brochothrix thermosphacta | |
0.25 | - |
D-fructose 6-phosphate | cosubstrate: NADH | Brochothrix thermosphacta |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Brochothrix thermosphacta | 5622 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
gel filtration | Brochothrix thermosphacta |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-mannitol 1-phosphate + NAD+ | Brochothrix thermosphacta | - |
D-fructose 6-phosphate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brochothrix thermosphacta | - |
formerly Microbacterium thermosphactum | - |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography techniques | Brochothrix thermosphacta |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | inducible by D-mannitol | Brochothrix thermosphacta | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.09 | - |
crude extract, D-mannitol 1-phosphate oxidation | Brochothrix thermosphacta |
18 | - |
purified enzyme, D-fructose 6-phosphate reduction | Brochothrix thermosphacta |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-mannitol 1-phosphate + NAD+ | - |
Brochothrix thermosphacta | D-fructose 6-phosphate + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 50000, SDS-PAGE | Brochothrix thermosphacta |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | oxidation of D-mannitol 1-phosphate | Brochothrix thermosphacta |
9.5 | 10 | reduction of D-fructose 6-phosphate | Brochothrix thermosphacta |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | higher affinity for NADH | Brochothrix thermosphacta | |
NADH | higher affinity than for NAD+ | Brochothrix thermosphacta |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
ADP | more potent inhibitor than ATP | Brochothrix thermosphacta | |
1.6 | - |
ATP | - |
Brochothrix thermosphacta |