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Literature summary for 1.1.1.17 extracted from

  • Singh, S.P.; Rogers, P.J.
    Isolation and characterization of mannitol-1-phosphate dehydrogenase from Brochothrix thermosphacta (1993), J. Gen. Appl. Microbiol., 39, 327-337.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
ADP competitive inhibition Brochothrix thermosphacta
ATP competitive inhibition Brochothrix thermosphacta

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
NADH higher affinity for NADH Brochothrix thermosphacta
0.05
-
NAD+
-
Brochothrix thermosphacta
0.05
-
D-mannitol 1-phosphate cosubstrate: NAD+ Brochothrix thermosphacta
0.25
-
D-fructose 6-phosphate cosubstrate: NADH Brochothrix thermosphacta

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
Brochothrix thermosphacta 5622
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
gel filtration Brochothrix thermosphacta

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-mannitol 1-phosphate + NAD+ Brochothrix thermosphacta
-
D-fructose 6-phosphate + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Brochothrix thermosphacta
-
formerly Microbacterium thermosphactum
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography techniques Brochothrix thermosphacta

Source Tissue

Source Tissue Comment Organism Textmining
cell culture inducible by D-mannitol Brochothrix thermosphacta
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.09
-
crude extract, D-mannitol 1-phosphate oxidation Brochothrix thermosphacta
18
-
purified enzyme, D-fructose 6-phosphate reduction Brochothrix thermosphacta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-mannitol 1-phosphate + NAD+
-
Brochothrix thermosphacta D-fructose 6-phosphate + NADH + H+
-
r

Subunits

Subunits Comment Organism
monomer 1 * 50000, SDS-PAGE Brochothrix thermosphacta

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7 oxidation of D-mannitol 1-phosphate Brochothrix thermosphacta
9.5 10 reduction of D-fructose 6-phosphate Brochothrix thermosphacta

Cofactor

Cofactor Comment Organism Structure
NAD+ higher affinity for NADH Brochothrix thermosphacta
NADH higher affinity than for NAD+ Brochothrix thermosphacta

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.7
-
ADP more potent inhibitor than ATP Brochothrix thermosphacta
1.6
-
ATP
-
Brochothrix thermosphacta