Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21/lambdaDE3 | Salmonella enterica subsp. enterica serovar Typhimurium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.742 | - |
ketopantoic acid | +/-0.01 | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.776 | - |
NADPH | +/-0.09 | Salmonella enterica subsp. enterica serovar Typhimurium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31100 | - |
HPLC gel filtration | Salmonella enterica subsp. enterica serovar Typhimurium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ketopantoate + NADPH | Escherichia coli | - |
pantoate + NADP+ | - |
? | |
ketopantoate + NADPH | Salmonella enterica subsp. enterica serovar Typhimurium | thiamine synthesis, pantothenate and thiamine biosynthetic pathway | pantoate + NADP+ | - |
? | |
ketopantoate + NADPH | Escherichia coli BL21/lambdaDE3 | - |
pantoate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
BL21/lambdaDE3 | - |
Escherichia coli BL21/lambdaDE3 | - |
BL21/lambdaDE3 | - |
Saccharomyces cerevisiae | - |
- |
- |
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Stenotrophomonas maltophilia | - |
845 | - |
Stenotrophomonas maltophilia 845 | - |
845 | - |
Purification (Comment) | Organism |
---|---|
chromatography | Salmonella enterica subsp. enterica serovar Typhimurium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Escherichia coli |
additional information | - |
- |
Saccharomyces cerevisiae |
additional information | - |
no activity with pantoate, ketoisovalerate, oxaloacetate, pyruvate, 3-hydroxypyruvic acid, alpha-ketoglutarate, alpha-ketobutyrate, acetaldehyde at 0.5 mM | Salmonella enterica subsp. enterica serovar Typhimurium |
16 | - |
optimal reaction conditions using NADPH | Salmonella enterica subsp. enterica serovar Typhimurium |
61 | - |
optimal reaction conditions using NADH | Salmonella enterica subsp. enterica serovar Typhimurium |
64.3 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ketopantoate + NADPH | - |
Escherichia coli | pantoate + NADP+ | - |
? | |
ketopantoate + NADPH | thiamine synthesis, pantothenate and thiamine biosynthetic pathway | Salmonella enterica subsp. enterica serovar Typhimurium | pantoate + NADP+ | - |
? | |
ketopantoate + NADPH | - |
Escherichia coli BL21/lambdaDE3 | pantoate + NADP+ | - |
? | |
ketopantoic acid + NADH | - |
Salmonella enterica subsp. enterica serovar Typhimurium | pantoic acid + NAD+ | - |
? | |
ketopantoic acid + NADPH | - |
Salmonella enterica subsp. enterica serovar Typhimurium | pantoic acid + NADP+ | - |
? | |
ketopantoic acid + NADPH | - |
Escherichia coli | pantoic acid + NADP+ | - |
? | |
ketopantoic acid + NADPH | - |
Saccharomyces cerevisiae | pantoic acid + NADP+ | - |
? | |
ketopantoic acid + NADPH | - |
Escherichia coli BL21/lambdaDE3 | pantoic acid + NADP+ | - |
? | |
additional information | - |
Escherichia coli | ? | - |
? | |
additional information | - |
Saccharomyces cerevisiae | ? | - |
? | |
additional information | no activity with pantoate, ketoisovalerate, oxaloacetate, pyruvate, 3-hydroxypyruvic acid, alpha-ketoglutarate, alpha-ketobutyrate, acetaldehyde at 0.5 mM | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
additional information | - |
Escherichia coli BL21/lambdaDE3 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Escherichia coli |
monomer | 1 * 31100, HPLC gel filtration | Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | - |
reduction of ketopantoic acid at pH 6.0 | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.3 | - |
reduction of pantoic acid at 42°C | Salmonella enterica subsp. enterica serovar Typhimurium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | - |
Stenotrophomonas maltophilia | |
additional information | in presence of NADPH the ketopantoic acid reductase does not reduce artificial dye electron acceptors | Salmonella enterica subsp. enterica serovar Typhimurium | |
NADH | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
NADH | - |
Saccharomyces cerevisiae | |
NADPH | - |
Escherichia coli | |
NADPH | - |
Saccharomyces cerevisiae | |
NADPH | - |
Stenotrophomonas maltophilia | |
NADPH | preferred | Salmonella enterica subsp. enterica serovar Typhimurium |