General Stability | Organism |
---|---|
NADP+ protects against heat inactivation or Rose bengal-sensitized photoinactivation and cold inactivation | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0068 | - |
NADPH | - |
Bos taurus | |
0.22 | - |
NADH | - |
Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22000 | - |
4 * 22000, SDS-PAGE | Bos taurus |
90000 | - |
sedimentation equilibrium analysis, gel filtration, sucrose density gradient centrifugation | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
123 | - |
- |
Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-erythrulose + NAD(P)H | highly specific for D-erythrulose | Bos taurus | D-threitol + NAD(P)+ | - |
ir | |
D-erythrulose + NAD(P)H | reversibility could not be demonstrated | Bos taurus | D-threitol + NAD(P)+ | - |
ir | |
D-erythrulose + NADH + H+ | - |
Bos taurus | D-threitol + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 22000, SDS-PAGE | Bos taurus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
cold inactivation | Bos taurus |
- |
- |
23.5 h, 90% loss of activity, regains about 55-65% of its original activity after 60 min | Bos taurus |
21 | - |
23.5 h, 20-25% loss of activity | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.85 | - |
D-erythrulose + NADH | Bos taurus |
6.25 | - |
D-erythrulose + NADH | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | utilized more slowly than NADPH | Bos taurus | |
NADPH | - |
Bos taurus |