Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | unidentified microorganism |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | magnesium ion significantly enhances activity and thermostability of the enzyme. The enzymatic activity increases 40fold in the presence of 50 mM Mg2+, and T0.5 increases by approximately 6°C | unidentified microorganism |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
unidentified microorganism | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
20 | - |
with ethyl benzoylacetate as substrate, at pH 10.5, temperature not specified in the publication | unidentified microorganism |
188.3 | - |
with taurochenodeoxycholic acid as substrate, at pH 10.5, temperature not specified in the publication | unidentified microorganism |
217.6 | - |
with glycochenodeoxycholic acid as substrate, at pH 10.5, temperature not specified in the publication | unidentified microorganism |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethyl benzoylacetate + NAD(P)+ | - |
unidentified microorganism | ? | - |
? | |
glycochenodeoxycholic acid + NAD(P)+ | - |
unidentified microorganism | ? | - |
? | |
taurochenodeoxycholic acid + NAD(P)+ | - |
unidentified microorganism | tauroursodeoxycholic acid + NAD(P)H + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
7alpha-HSDH | - |
unidentified microorganism |
J-1-1 | - |
unidentified microorganism |
NADP(H)-dependent 7alpha-HSDH | - |
unidentified microorganism |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
73% of the enzyme activity is maintained after heat treatment at 40°C for 100 h | unidentified microorganism |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
unidentified microorganism | |
NADP+ | - |
unidentified microorganism |