Cloned (Comment) | Organism |
---|---|
recombinant expression of wild-type and truncation mutant enzymes in Escherichia coli | Clostridium sardiniense |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of a C-terminaly truncation enzyme mutant. When 2 and 6 amino acids of C-terminal are removed, the catalytic efficiency of Ca7alpha-HSDH remains 19.1% and 2.5%, respectively. No activity remains after deletion of 8, 14 and 17 amino acids | Clostridium sardiniense |
R38D | site-directed mutagenesis, inactive mutant | Clostridium sardiniense |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | activates by 105.7% at 500 mM | Clostridium sardiniense | |
Mg2+ | activates by 61.4% at 50 mM | Clostridium sardiniense | |
Na+ | activates by 64.7% at 200 mM | Clostridium sardiniense |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholate + NAD+ | Clostridium sardiniense | - |
3alpha,12alpha-dihydroxy-7-oxo-5beta-cholanate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium sardiniense | G9FRD7 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholate + NAD+ | - |
Clostridium sardiniense | 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholanate + NADH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
7alpha-HSDH | - |
Clostridium sardiniense |
Ca7alpha-HSDH | - |
Clostridium sardiniense |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 37 | purified recombinant enzyme, no change in activity after incubation at 4°C or 25°C for 108 h, but dramatic decrease in activity at 37°C | Clostridium sardiniense |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Clostridium sardiniense |
General Information | Comment | Organism |
---|---|---|
evolution | 7alpha-hydroxysteroid dehydrogenase is a member of the short-chain dehydrogenase/reductase superfamily | Clostridium sardiniense |
additional information | the C-terminus and residue Arg38 are essential for activity of the 7alpha-hydroxysteroid dehydrogenase from Clostridium absonum | Clostridium sardiniense |
physiological function | the enzyme catalyzes the dehydrogenation of C7 hydroxyl group of the steroid skeleton of bile acids | Clostridium sardiniense |