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Literature summary for 1.1.1.159 extracted from

  • Giovannini, P.P.; Grandini, A.; Perrone, D.; Pedrini, P.; Fantin, G.; Fogagnolo, M.
    7alpha- and 12alpha-Hydroxysteroid dehydrogenases from Acinetobacter calcoaceticus lwoffii: a new integrated chemo-enzymatic route to ursodeoxycholic acid (2008), Steroids, 73, 1385-1390.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
NAD+
-
Acinetobacter lwoffii

Application

Application Comment Organism
degradation new integrated chemo-enzymatic synthesis of ursodeoxycholic acid starting from sodium cholate by 7alpha- and 12alpha-hydroxysteroid dehydrogenases Acinetobacter lwoffii

Organism

Organism UniProt Comment Textmining
Acinetobacter lwoffii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partially purified by ion-exchange chromatography Acinetobacter lwoffii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.035
-
cell extract Acinetobacter lwoffii
0.11
-
purified enzyme Acinetobacter lwoffii

Storage Stability

Storage Stability Organism
4°C, 7 days Acinetobacter lwoffii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chenodeoxycholic acid + NAD+
-
Acinetobacter lwoffii 3alpha-hydroxy-7-oxo-5beta-cholan-24-oic acid + NADH
-
?

Synonyms

Synonyms Comment Organism
7alpha-HSDH
-
Acinetobacter lwoffii