Application | Comment | Organism |
---|---|---|
synthesis | the enzyme is useful as biocatalyst in the reduction of 7-keto bile acids, co-operation with cholylglycine hydrolase, EC 3.5.1.24, overview | Stenotrophomonas maltophilia |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
NAD+ | pH 7.5, 22°C | Stenotrophomonas maltophilia | |
1 | - |
cholic acid | pH 7.5, 22°C | Stenotrophomonas maltophilia |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Stenotrophomonas maltophilia | substrate specificity and cycles in bile acid pathways, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Stenotrophomonas maltophilia | - |
- |
- |
Stenotrophomonas maltophilia CBS 897.97 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 175fold by anion exchange chromatography, ammonium sulfate fractionation, hydrophobic interaction and affinity chromatography | Stenotrophomonas maltophilia |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Stenotrophomonas maltophilia |
70 | - |
purified native enzyme | Stenotrophomonas maltophilia |
Storage Stability | Organism |
---|---|
4°C, purified enzyme, 50 mM sodium phosphate, pH 7.5, stable for 2 days | Stenotrophomonas maltophilia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3alpha,5beta)-3-hydroxy-7,12-dioxocholan-24-oic acid + NADH | - |
Stenotrophomonas maltophilia | (3alpha,5beta,7alpha)-3,7-dihydroxy-12-oxocholan-24-oic acid + NAD+ | - |
r | |
3,7-diketocholic acid + NADH | high activity | Stenotrophomonas maltophilia | 3-ketocholic acid + NAD+ | - |
r | |
6-fluoro-7-ketohyocholic acid + NADH | - |
Stenotrophomonas maltophilia | 6-fluoro-3alpha,6alpha,7alpha-trihydroxy-5beta-cholan-24-oic acid + NAD+ + NAD+ | - |
r | |
7-ketochenodeoxycholic acid + NADH | - |
Stenotrophomonas maltophilia | 3alpha,7alpha-dihydroxy-5beta-cholanoic acid + NAD+ | - |
r | |
7-ketohyocholic acid + NADH | low activity | Stenotrophomonas maltophilia | 3alpha,6alpha,7alpha-trihydroxy-5beta-cholan-24-oic acid + NAD+ | - |
r | |
cholic acid + NADH | low activity in the reverse reaction | Stenotrophomonas maltophilia | 7-ketocholic acid + NAD+ | - |
r | |
additional information | substrate specificity and cycles in bile acid pathways, overview | Stenotrophomonas maltophilia | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
7alpha-HSDH | - |
Stenotrophomonas maltophilia |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
substrate cholic acid | Stenotrophomonas maltophilia |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Stenotrophomonas maltophilia |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Stenotrophomonas maltophilia | |
NADH | - |
Stenotrophomonas maltophilia |