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Literature summary for 1.1.1.159 extracted from
- Cloning and sequencing of the 7alpha-hydroxysteroid dehydrogenase gene from Escherichia coli HB101 and characterization of the expressed enzyme (1991), J. Bacteriol., 173, 2173-2179.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene from strain HB101 cloned in plasmid pSD1 and expressed in Escherichia coli strain DH1 | Escherichia coli |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CoCl2 | nearly complete inhibition | Escherichia coli |  |
| CuCl2 | nearly complete inhibition | Escherichia coli | |
| diethyl dicarbonate | complete | Escherichia coli | |
| FeCl2 | - |
Escherichia coli | |
| FeCl3 | nearly complete inhibition | Escherichia coli | |
| HgCl2 | - |
Escherichia coli | |
| iodoacetic acid | 70% remaining activity at concentration 1 mM | Escherichia coli | |
| N-bromosuccinimide | complete | Escherichia coli | |
| NiSO4 | - |
Escherichia coli | |
| o-phenanthroline | 70% remaining acitvity at concentration 1 mM | Escherichia coli | |
| p-chloromercuribenzoate | 80% remaining activity at concentration 0.3 mM | Escherichia coli | |
| ZnCl2 | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.19 | - |
taurochenodeoxycholic acid | - |
Escherichia coli | |
| 0.43 | - |
chenodeoxycholic acid | - |
Escherichia coli | |
| 1.2 | - |
cholic acid | - |
Escherichia coli | |
| 1.25 | - |
glycocholic acid | - |
Escherichia coli | |
| 2 | - |
taurocholic acid | - |
Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
4 * 28000, SDS-PAGE | Escherichia coli |
| 120000 | - |
gel filtration | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant from Escherichia coli, high yield | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chenodeoxycholic acid + NAD(P)+ | - |
Escherichia coli | 3alpha-hydroxy-7-oxo-5beta-cholan-24-oic acid + NAD(P)H | - |
? | |
| cholic acid + NAD(P)+ | - |
Escherichia coli | 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholan-24-oic acid + NADH | - |
? | |
| glycocholic acid + NAD+ | - |
Escherichia coli | N-[3alpha,12alpha-dihydroxy-7,24-dioxo-5beta-cholan-24-yl]glycine + NADH | - |
? | |
| additional information | no activity with deoxycholic acid and taurodeoxycholic acid | Escherichia coli | ? | - |
? | |
| taurochenodeoxycholic acid + NAD+ | - |
Escherichia coli | 2[[3alpha-hydroxy-7,24-dioxo-5beta-cholan-24-yl]amino]ethane sulfonic acid + NADH | - |
? | |
| taurocholic acid + NAD+ | - |
Escherichia coli | 2[[3alpha,12alpha-dihydroxy-7,24-dioxo-5beta-cholan-24-yl]amino]ethane sulfonic acid + NADH | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 28000, SDS-PAGE | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.417 | - |
glycocholic acid | - |
Escherichia coli | |
| 3.13 | - |
taurocholic acid | - |
Escherichia coli | |
| 3.43 | - |
taurochenodeoxycholic acid | - |
Escherichia coli | |
| 8.18 | - |
chenodeoxycholic acid | - |
Escherichia coli | |
| 63.3 | - |
cholic acid | - |
Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Escherichia coli |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9 | - |
Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Escherichia coli | |
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