General Stability | Organism |
---|---|
inactivation by freezing | Escherichia coli |
stabilization by glutathione, EDTA | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
chenodeoxycholate | substrate inhibition at concentration above 1 mM | Escherichia coli | |
glycochenodeoxycholate | substrate inhibition at concentration above 1 mM | Escherichia coli | |
taurochenodeoxycholate | substrate inhibition at concentration above 1 mM | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
chenodeoxycholic acid | - |
Escherichia coli | |
0.085 | - |
glycochenodeoxycholic acid | - |
Escherichia coli | |
0.24 | - |
taurochenodeoxycholic acid | - |
Escherichia coli | |
0.8 | - |
cholic acid | - |
Escherichia coli | |
1 | - |
taurocholic acid | - |
Escherichia coli | |
1 | - |
glycocholic acid | - |
Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
bile acids + NAD(P)+ | Escherichia coli | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
bile acids + NAD(P)+ | - |
Escherichia coli | ? | - |
? | |
chenodeoxycholic acid + NAD(P)+ | i.e. 3alpha,7alpha-dihydroxy-5beta-cholan-24-oic acid | Escherichia coli | 3alpha-hydroxy-7-oxo-5beta-cholan-24-oic acid + NAD(P)H | - |
? | |
cholic acid + NAD(P)+ | - |
Escherichia coli | 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholan-24-oic acid + NADH | - |
? | |
glycochenodeoxycholic acid + NAD(P)+ | - |
Escherichia coli | N-[3alpha-hydroxy-7,24-dioxocholan-24-yl]glycine + NAD(P)H | - |
? | |
glycocholic acid + NAD+ | i.e. N-[3alpha,7alpha,12alpha-trihydroxy-24-oxocholan-24-yl]glycine | Escherichia coli | N-[3alpha,12alpha-dihydroxy-7,24-dioxo-5beta-cholan-24-yl]glycine + NADH | - |
? | |
taurochenodeoxycholic acid + NAD+ | - |
Escherichia coli | 2[[3alpha-hydroxy-7,24-dioxo-5beta-cholan-24-yl]amino]ethane sulfonic acid + NADH | - |
? | |
taurocholic acid + NAD+ | i.e. 2[[3alpha,7alpha,12alpha-trihydroxy-24-oxo-5beta-cholan-24-yl]amino]ethane sulfonic acid | Escherichia coli | 2[[3alpha,12alpha-dihydroxy-7,24-dioxo-5beta-cholan-24-yl]amino]ethane sulfonic acid + NADH | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | 10 | oxidation of cholic acid | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Escherichia coli |