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Literature summary for 1.1.1.159 extracted from

  • MacDonald, I.A.; Williams, C.N.; Mahony, D.E.
    7alpha-hydroxysteroid dehydrogenase from Escherichia coli B: preliminary studies (1973), Biochim. Biophys. Acta, 309, 243-253.
    View publication on PubMed

General Stability

General Stability Organism
inactivation by freezing Escherichia coli
stabilization by glutathione, EDTA Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
chenodeoxycholate substrate inhibition at concentration above 1 mM Escherichia coli
glycochenodeoxycholate substrate inhibition at concentration above 1 mM Escherichia coli
taurochenodeoxycholate substrate inhibition at concentration above 1 mM Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
chenodeoxycholic acid
-
Escherichia coli
0.085
-
glycochenodeoxycholic acid
-
Escherichia coli
0.24
-
taurochenodeoxycholic acid
-
Escherichia coli
0.8
-
cholic acid
-
Escherichia coli
1
-
taurocholic acid
-
Escherichia coli
1
-
glycocholic acid
-
Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
bile acids + NAD(P)+ Escherichia coli
-
?
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bile acids + NAD(P)+
-
Escherichia coli ?
-
?
chenodeoxycholic acid + NAD(P)+ i.e. 3alpha,7alpha-dihydroxy-5beta-cholan-24-oic acid Escherichia coli 3alpha-hydroxy-7-oxo-5beta-cholan-24-oic acid + NAD(P)H
-
?
cholic acid + NAD(P)+
-
Escherichia coli 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholan-24-oic acid + NADH
-
?
glycochenodeoxycholic acid + NAD(P)+
-
Escherichia coli N-[3alpha-hydroxy-7,24-dioxocholan-24-yl]glycine + NAD(P)H
-
?
glycocholic acid + NAD+ i.e. N-[3alpha,7alpha,12alpha-trihydroxy-24-oxocholan-24-yl]glycine Escherichia coli N-[3alpha,12alpha-dihydroxy-7,24-dioxo-5beta-cholan-24-yl]glycine + NADH
-
?
taurochenodeoxycholic acid + NAD+
-
Escherichia coli 2[[3alpha-hydroxy-7,24-dioxo-5beta-cholan-24-yl]amino]ethane sulfonic acid + NADH
-
?
taurocholic acid + NAD+ i.e. 2[[3alpha,7alpha,12alpha-trihydroxy-24-oxo-5beta-cholan-24-yl]amino]ethane sulfonic acid Escherichia coli 2[[3alpha,12alpha-dihydroxy-7,24-dioxo-5beta-cholan-24-yl]amino]ethane sulfonic acid + NADH
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9 10 oxidation of cholic acid Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Escherichia coli