General Stability | Organism |
---|---|
NAD+-dependent form lyophilized is stable to freezing/thawing, NADP+-dependent form lyophilized is unstable | Bacteroides fragilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
3alpha,7alpha-Dihydroxy-5beta-cholanoyl glycine | - |
Bacteroides fragilis | |
0.1 | - |
3alpha,7alpha-dihydroxy-5beta-cholanoic acid | - |
Bacteroides fragilis | |
0.1 | - |
3alpha,7alpha-dihydroxy-5beta-cholanoyl taurine | - |
Bacteroides fragilis | |
0.32 | 0.34 | 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoyl glycine | - |
Bacteroides fragilis | |
0.32 | 0.34 | 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoic acid | - |
Bacteroides fragilis | |
0.32 | 0.34 | 3alpha,7alpha,12alpha-trihydroxycholanoyl taurine | - |
Bacteroides fragilis | |
0.45 | - |
NAD+ | with chenodeoxycholic acid | Bacteroides fragilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacteroides fragilis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3alpha,7alpha,12alpha-trihydroxycholanoyl taurine + NAD+ | - |
Bacteroides fragilis | 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholanoyl taurine NADH + H+ + | - |
r | |
3alpha,7alpha-dihydroxy-5beta-cholanoyl taurine + NAD+ | i.e. chenodeoxycholic acid | Bacteroides fragilis | 3alpha-hydroxy-7-oxo-5beta-cholanoyl taurine + NADH + H+ | - |
r | |
chenodeoxycholic acid + NAD(P)+ | i.e. 3alpha,7alpha-dihydroxy-5beta-cholan-24-oic acid | Bacteroides fragilis | 3alpha-hydroxy-7-oxo-5beta-cholan-24-oic acid + NAD(P)H | - |
? | |
glycochenodeoxycholic acid + NAD(P)+ | - |
Bacteroides fragilis | N-[3alpha-hydroxy-7,24-dioxocholan-24-yl]glycine + NAD(P)H | - |
? | |
glycocholic acid + NAD+ | - |
Bacteroides fragilis | N-[3alpha,12alpha-dihydroxy-7,24-dioxo-5beta-cholan-24-yl]glycine + NADH | - |
? | |
taurochenodeoxycholic acid + NAD+ | - |
Bacteroides fragilis | 2[[3alpha-hydroxy-7,24-dioxo-5beta-cholan-24-yl]amino]ethane sulfonic acid + NADH | - |
? | |
taurocholic acid + NAD+ | i.e. 2[[3alpha,7alpha,12alpha-trihydroxy-24-oxo-5beta-cholan-24-yl]amino]ethane sulfonic acid | Bacteroides fragilis | 2[[3alpha,12alpha-dihydroxy-7,24-dioxo-5beta-cholan-24-yl]amino]ethane sulfonic acid + NADH | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | NADP+-dependent activity | Bacteroides fragilis |
9.5 | 10 | NAD+-dependent activity | Bacteroides fragilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Bacteroides fragilis | |
NADP+ | - |
Bacteroides fragilis |