Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Bacteroides fragilis | |
EDTA | - |
Bacteroides fragilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activation | Bacteroides fragilis | |
MnCl2 | activation | Bacteroides fragilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
NAD+-specific form, gel filtration | Bacteroides fragilis |
127000 | - |
NADP+-dependent form, gel filtration | Bacteroides fragilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
bile acids + NAD(P)+ | Bacteroides fragilis | serve as energy sources in absence of glucose | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacteroides fragilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Bacteroides fragilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
bile acids + NAD(P)+ | serve as energy sources in absence of glucose | Bacteroides fragilis | ? | - |
? | |
chenodeoxycholic acid + NAD(P)+ | preferred substrate with NAD+ as coenzyme | Bacteroides fragilis | 3alpha-hydroxy-7-oxo-5beta-cholan-24-oic acid + NAD(P)H | - |
? | |
chenodeoxycholic acid + NAD(P)+ | i.e. 3alpha,7alpha-dihydroxy-5beta-cholan-24-oic acid | Bacteroides fragilis | 3alpha-hydroxy-7-oxo-5beta-cholan-24-oic acid + NAD(P)H | - |
? | |
cholic acid + NAD(P)+ | preferred substrate with NADP+ as coenzyme | Bacteroides fragilis | 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholan-24-oic acid + NADH | - |
? | |
cholic acid + NAD(P)+ | i.e. 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate | Bacteroides fragilis | 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholan-24-oic acid + NADH | - |
? | |
glycochenodeoxycholic acid + NAD(P)+ | - |
Bacteroides fragilis | N-[3alpha-hydroxy-7,24-dioxocholan-24-yl]glycine + NAD(P)H | - |
? | |
glycocholic acid + NAD+ | i.e. N-[3alpha,7alpha,12alpha-trihydroxy-24-oxocholan-24-yl]glycine | Bacteroides fragilis | N-[3alpha,12alpha-dihydroxy-7,24-dioxo-5beta-cholan-24-yl]glycine + NADH | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
inactivation of NADP+-dependent form, NAD+-dependent form stable | Bacteroides fragilis |
75 | - |
30 min, presence of substrates, NAD+-dependent form stable | Bacteroides fragilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9 | oxidation of 7alpha-hydroxy group | Bacteroides fragilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | one enzyme form specific for NAD+, other form specific for NADP+ | Bacteroides fragilis | |
NADP+ | one enzyme form specific for NADP+, other form specific for NAD+ | Bacteroides fragilis |