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Literature summary for 1.1.1.157 extracted from
- Simultaneous single-step purification of thiolase and NADP-dependent 3-hydroxybutyryl-CoA dehydrogenase from Clostridium kluyveri (1984), Anal. Biochem., 141, 344-347.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33500 | - |
x * 33500, SDS-PAGE | Clostridium kluyveri |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-acetoacetyl-CoA + NADPH + H+ | Clostridium kluyveri | - |
(S)-3-hydroxybutanoyl-CoA + NADP+ | - |
r |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium kluyveri | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| simultaneous single-step purification of thiolase and NADP-dependent 3-hydroxybutyryl-CoA dehydrogenase | Clostridium kluyveri |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 292 | - |
pH 6.5, 25°C | Clostridium kluyveri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-acetoacetyl-CoA + NADPH + H+ | - |
Clostridium kluyveri | (S)-3-hydroxybutanoyl-CoA + NADP+ | - |
r | |
| 3-acetoacetyl-CoA + NADPH + H+ | NADPH-dependent enzyme specific for 3-hydroxybutyryl-CoA | Clostridium kluyveri | (S)-3-hydroxybutanoyl-CoA + NADP+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 33500, SDS-PAGE | Clostridium kluyveri |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Clostridium kluyveri |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
assay at | Clostridium kluyveri |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | specific for NADPH | Clostridium kluyveri | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Clostridium kluyveri | isoelectric focusing, pH 3.5-10.0 | - |
7.8 |
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