Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.153 extracted from

  • Meng, Y.; Katsuma, S.; Daimon, T.; Banno, Y.; Uchino, K.; Sezutsu, H.; Tamura, T.; Mita, K.; Shimada, T.
    The silkworm mutant lemon (lemon lethal) is a potential insect model for human sepiapterin reductase deficiency (2009), J. Biol. Chem., 284, 11698-11705.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) cells Bombyx mori

Inhibitors

Inhibitors Comment Organism Structure
Melatonin
-
Bombyx mori
N-acetylserotonin
-
Bombyx mori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0283
-
sepiapterin recombinant enzyme, in 100 mM potassium phosphate buffer (pH 6.4), at 37°C Bombyx mori

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29200
-
calculated from amino acid sequence Bombyx mori

Organism

Organism UniProt Comment Textmining
Bombyx mori
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His GraviTrap nickel affinity column chromatography, gel filtration Bombyx mori

Source Tissue

Source Tissue Comment Organism Textmining
larva
-
Bombyx mori
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sepiapterin + NADPH + H+
-
Bombyx mori 7,8-dihydrobiopterin + NADP+
-
?

Synonyms

Synonyms Comment Organism
SPR
-
Bombyx mori

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Bombyx mori

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Bombyx mori

pH Range

pH Minimum pH Maximum Comment Organism
4 6
-
Bombyx mori

Cofactor

Cofactor Comment Organism Structure
NADPH NADPH-dependent enzyme Bombyx mori

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.1
-
recombinant enzyme, in 100 mM potassium phosphate buffer (pH 6.4), at 37°C Bombyx mori Melatonin
0.2
-
recombinant enzyme, in 100 mM potassium phosphate buffer (pH 6.4), at 37°C Bombyx mori N-acetylserotonin