Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cibacron Blue 3G-A | IC50 0.01 mM | Rattus norvegicus | |
dihydrobiopterin | non-competitive with NADPH or sepiapterin as varying substrate | Rattus norvegicus | |
NADP+ | competitive inhibition at fixed concentration with varied NADPH, noncompetitive with varied sepiapterin | Rattus norvegicus | |
NADPH | product inhibition, with more than 0.1 mM, with 0.05 mM sepiapterin | Rattus norvegicus | |
sepiapterin | product inhibition, with more than 0.05 mM, with 0.1 mM NADPH | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0017 | - |
NADPH | - |
Rattus norvegicus | |
0.015 | - |
sepiapterin | - |
Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27500 | - |
2 * 27500, SDS-PAGE | Rattus norvegicus |
55000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-(S)-lactoyl-7,8-dihydropterin + NADPH | Rattus norvegicus | equilibrium lies much in favor of dihydrobiopterin formation | 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+ | - |
? | |
6-(S)-lactoyl-7,8-dihydropterin + NADPH | Rattus norvegicus | terminal step in the biosynthetic pathway for tetrahydrobiopterin | 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-erythro-7,8-dihydrobiopterin + NADP+ = sepiapterin + NADPH + H+ | ordered bi-bi mechanism, NADPH binds first and NADP+ releases last | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
erythrocyte | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
18 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
-80°C, in 50 mM potassium phosphate buffer, 0,1 M KCl, pH 6.8 | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-(S)-lactoyl-7,8-dihydropterin + NADPH | equilibrium lies much in favor of dihydrobiopterin formation | Rattus norvegicus | 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+ | - |
? | |
6-(S)-lactoyl-7,8-dihydropterin + NADPH | terminal step in the biosynthetic pathway for tetrahydrobiopterin | Rattus norvegicus | 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 27500, SDS-PAGE | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
in potassium phosphate buffer | Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7.4 | pH 5.5: optimum, pH 7.4: in 50 mM Tris-HCl buffer about 80% of maximum activity observed | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Rattus norvegicus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.01 | - |
IC50 0.01 mM | Rattus norvegicus | Cibacron Blue 3G-A |