Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.149 extracted from

  • Ma, H.; Penning, T.M.
    Characterization of homogeneous recombinant rat ovarian 20alpha-hydroxysteroid dehydrogenase: fluorescent properties and inhibition profile (1999), Biochem. J., 341, 853-859.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.149

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli C41 (DE3) Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
Flufenamic acid poor inhibition for 20alpha-hydroxyprogesterone oxidation Rattus norvegicus
Meclofenamic acid 20alpha-hydroxyprogesterone oxidation Rattus norvegicus
additional information not inhibited by indomethacin, tolmetin and steroidal anti-inflammatory drugs Rattus norvegicus
synthetic non-steroidal oestrogens hexoestrol most potent competitive inhibitor Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00195
-
 9,10-phenanthrenequinone +/-0.0004 Rattus norvegicus
0.008
-
 NADP+ +/-0.0011 Rattus norvegicus
0.009
-
 20alpha-hydroxyprogesterone +/-0.0001 Rattus norvegicus
0.011
-
 progesterone +/-0.0017 Rattus norvegicus
0.012
-
 3alpha-hydroxy-5alpha-pregnan-20-one +/-0.0035 Rattus norvegicus
0.013
-
 20alpha-hydroxy-5alpha-pregnan-3-one +/-0.0035 Rattus norvegicus
0.014
-
 NADPH +/-0.004 Rattus norvegicus
0.0154
-
 17alpha-hydroxyprogesterone +/-0.0029 Rattus norvegicus
0.3811
-
 4-nitrobenzaldehyde +/-0.0605 Rattus norvegicus
120
-
 D,L-glyceraldehyde
-
 Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
 SDS-PAGE Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 female, Sprague-Dawley, 2 weeks
-

Purification (Commentary)

Purification (Comment) Organism
anion exchange and affinity chromatography Rattus norvegicus

Reaction

Reaction Comment Organism Reaction ID
17alpha,20alpha-dihydroxypregn-4-en-3-one + NAD(P)+ = 17alpha-hydroxyprogesterone + NAD(P)H + H+ bibi mechanism Rattus norvegicus
a

Source Tissue

Source Tissue Comment Organism Textmining
ovary
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.002
-
 reduction of 0.06 mM prostaglandin E2 Rattus norvegicus
0.007
-
 reduction of 0.03 mM 17alpha-hydroxy-pregnenolone Rattus norvegicus
0.013
-
 reduction of 100 mM D-glucose Rattus norvegicus
0.016
-
 reduction of 0.03 mM prednisolone Rattus norvegicus
0.017
-
 reduction of 100 mM D-galactose Rattus norvegicus
0.025
-
 reduction of 0.03 mM prednisone Rattus norvegicus
0.067
-
 reduction of 0.05 mM pregnenolone Rattus norvegicus
0.9
-
 reduction of 0.06 mM 5alpha-pregnane-3,20-dione Rattus norvegicus
2.09
-
 20alpha-hydroxyprogesterone Rattus norvegicus
2.5
-
 oxidation of 0.06 mM 20alpha-hydroxy-5beta-pregnan-3-one Rattus norvegicus

Storage Stability

Storage Stability Organism
-70°C, 2.8 mg/ml protein concentration, 20 mM potassium phosphate buffer, pH 7.0, 1 mM EDTA, 1 mM beta-mercaptoethanol, 30%, v/v, glycerol Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
17alpha-hydroxypregnenolone + NADPH
-
 Rattus norvegicus ? + NADP+
-
 ?
17alpha-hydroxyprogesterone + NADPH + H+
-
 Rattus norvegicus 17alpha,20alpha-dihydroxy-pregn-4-en-3-one + NADP+
-
 ?
20alpha-hydroxy-5alpha-pregnan-3-one + NADPH + H+
-
 Rattus norvegicus 5alpha-pregnan-20alpha,3alpha-diol + NADP+
-
 r
3alpha-hydroxy-5-alpha-pregnan-20-one + NADPH + H+
-
 Rattus norvegicus 5alpha-pregnan-3alpha,20alpha-diol + NADP+
-
 r
3alpha-hydroxy-5alpha-pregnan-20-one + NADPH + H+
-
 Rattus norvegicus 5alpha-pregnan-3alpha,20alpha-diol + NADP+
-
 r
4-nitrobenzaldehyde + NADPH
-
 Rattus norvegicus 4-nitrobenzyl alcohol + NADP+
-
 ?
9,10-phenanthrenequinone + NADPH
-
 Rattus norvegicus NADP+ + ?
-
 ?
DL-glyceraldehyde + NADPH
-
 Rattus norvegicus glycerol + NADP+
-
 ?
additional information high activity for neuroactive steroids, 9,10-phenanthrenequinone and 4-nitrobenzaldehyde, low activity for 17alpha hydroxypregnenolone, D,L-glyceraldehyde and prostaglandin E2, no activity for 20beta-hydroxy-pregn-4-en-3-one, andro-, testosterone, 17alpha-, 17beta-oestradiol, oestrone and 5alpha-dihydrotestosterone, other substrates: DL-glyceraldehyde, prostaglandin E2, D-glucose, prednisolone, D-galactose, prednisone, pregnenolone, 5alpha-pregnane-3,20-dione, 9,10-phenanthrenequinone Rattus norvegicus ?
-
 ?
progesterone + NADPH
-
 Rattus norvegicus 20alpha-hydroxyprogesterone + NADP+
-
 r
prostaglandin E2 + NADPH low activity Rattus norvegicus NADP+ + ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 37000, SDS-PAGE Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.178
-
 17alpha-hydroxyprogesterone +/-0.8 Rattus norvegicus
0.208
-
 3alpha-hydroxy-5-alpha-pregnan-20-one +/-1.6 Rattus norvegicus
0.215
-
 D,L-glyceraldehyde +/-0.3 Rattus norvegicus
0.785
-
 20alpha-hydroxy-5alpha-pregnan-3-one +/-4.3 Rattus norvegicus
1.17
-
 20alpha-hydroxyprogesterone +/-0.2 Rattus norvegicus
1.43
-
 NADPH +/-1.3 Rattus norvegicus
1.52
-
 NADP+ +/-6.1 Rattus norvegicus
1.65
-
 progesterone +/-6.7 Rattus norvegicus
5.91
-
 4-nitrobenzaldehyde +/-33.4 Rattus norvegicus
7.7
-
 9,10-phenanthrenequinone +/-26.9 Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
NADP+ strictly specific Rattus norvegicus
NADPH strictly specific Rattus norvegicus