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Literature summary for 1.1.1.149 extracted from

  • Pineda, J.A.; Salinas, M.E.; Warren, J.C.
    Purification and characterization of 20alpha-hydroxysteroid dehydrogenase from bull testis (1985), J. Steroid Biochem., 23, 1001-1006.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.073
-
17-hydroxyprogesterone
-
Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
gel filtration Bos taurus
40000
-
SDS-PAGE Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, affinity chromatography, gel filtration Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
testis
-
Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.115
-
-
Bos taurus

Storage Stability

Storage Stability Organism
4°C, in presence of glycerol and DTT Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
17alpha-hydroxyprogesterone + NAD(P)H + H+ NADPH preferred Bos taurus 17alpha,20alpha-dihydroxy-pregn-4-en-3-one + NAD(P)+
-
?
additional information androstenedione and corticosterone not substrates Bos taurus ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 40000 SDS-PAGE Bos taurus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
stable in glycerol and dithiothreitol Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6
-
steroid reduction Bos taurus

Cofactor

Cofactor Comment Organism Structure
NADH
-
Bos taurus
NADPH preferred Bos taurus