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Literature summary for 1.1.1.149 extracted from
- 20alpha-Hydroxysteroid dehydrogenase of porcine testes (1972), J. Biol. Chem., 247, 815-823.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cysteine | restoring, also other sulfhydryl compounds, NADH-linked to a greater extent than the NADPH-linked activity | Sus scrofa |  |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Sus scrofa |
General Stability
| General Stability | Organism |
|---|---|
| significant activity loss by repeated thawing and freezing | Sus scrofa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | very powerful | Sus scrofa | |
| Cu2+ | very powerful | Sus scrofa | |
| Metal ions | strong | Sus scrofa | |
| additional information | Ca2+, Fe2+, Mg2+, Mn2+, Cd2+ and Zn2+ no significant effect | Sus scrofa | |
| p-chloromercuribenzoate | also other sufhydryl reagents like 5,5'-dithiobis-2-nitrobenzoic acid and iodoacetamide strong | Sus scrofa | |
| pyridoxal 5'-phosphate | strong, 90% inactivation by 10 mM | Sus scrofa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.022 | - |
NADPH | 10 mM cysteine, at 4°C until activity decreases to half of the initial value | Sus scrofa | |
| 0.052 | - |
NADPH | 10 mM cysteine | Sus scrofa | |
| 0.75 | - |
NADH | 10 mM cysteine | Sus scrofa | |
| 0.75 | - |
NADH | freshly thawed enzyme | Sus scrofa | |
| 1.1 | - |
NADH | 10 mM cysteine, at 4°C until activity decreases to half of the initial value | Sus scrofa | |
| 1.1 | - |
NADH | enzyme solution that has been left standing at 4°C until the activity has decreased to half of the initial value | Sus scrofa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
gel filtration, sedimentation, sucrose density gradient | Sus scrofa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sus scrofa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| six step procedure, ammonium sulfate fractionation, chromatography, gel filtration | Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| testis | - |
Sus scrofa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.081 | - |
- |
Sus scrofa |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, porcine testes, 0.1 M phosphate buffer, pH 7.00.1 M phosphate buffer, pH 7.0, several months | Sus scrofa |
| 4 or -20°C, 2 mg/ml purified protein, 0.1 M phosphate buffer, pH 6-9, several days or for months | Sus scrofa |
| 4°C or -20°C, ammonium sulfate fraction of purification step 2, over night or several months | Sus scrofa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 17alpha-hydroxy-progesterone + NADH + H+ | - |
Sus scrofa | 17alpha,20alpha-dihydroxypregn-4-en-3-one + NAD+ | - |
r | |
| 17alpha-hydroxycorticosterone + NADPH | less reactive than with 17alpha-hydroxyrogesterone | Sus scrofa | ? + NADP+ | - |
? | |
| 17alpha-hydroxyprogesterone + NAD(P)H + H+ | - |
Sus scrofa | 17alpha,20alpha-dihydroxy-pregn-4-en-3-one + NAD(P)+ | - |
r | |
| corticosterone + NADPH + H+ | less reactive than with 17alpha-hydroxyprogesterone | Sus scrofa | ? + NADP+ | - |
? | |
| additional information | no activity toward estrone with NADPH | Sus scrofa | ? | - |
? | |
| progesterone + NADPH | less reactive than with 17alpha-hydroxyprogesterone | Sus scrofa | 20alpha-hydroxyprogesterone + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Sus scrofa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
reduction of 17alpha-hydroxyprogesterone | Sus scrofa |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
55 | 10% of maximal reaction rate at 0°C | Sus scrofa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | - |
at 4°C more stable during purification than at 25°C | Sus scrofa |
| 25 | - |
unstable | Sus scrofa |
| 55 | - |
rapidly inactivated | Sus scrofa |
| 60 | - |
10 min, pH 5.5, complete inactivation of activity of purification step 1 and 2 | Sus scrofa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 7.5 | NADPH | Sus scrofa |
| 6.8 | 7.2 | NADH | Sus scrofa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | - |
Sus scrofa |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.2 | - |
activity destroyed below | Sus scrofa |
| 5.5 | - |
at pH 5.5 for 30 min, after first purification step | Sus scrofa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Sus scrofa | |
| NADPH | - |
Sus scrofa | |
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