Cloned (Comment) | Organism |
---|---|
expression in HEK293 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
E25K | 88% of wild type cortisone reduction activity | Homo sapiens |
E25K/E26K | 20% of wild type cortisone reduction activity | Homo sapiens |
E25K/E26Q | 45% of wild type cortisone reduction activity | Homo sapiens |
E25Q | 82% of wild type cortisone reduction activity | Homo sapiens |
E25Q/E26Q | 57% of wild type cortisone reduction activity | Homo sapiens |
E26K | 65% of wild type cortisone reduction activity | Homo sapiens |
K35S/K36S | no activity | Homo sapiens |
K5S/K6S | 93% of wild type cortisone reduction activity, no reduction of 7-oxocholesterol in intact HEK-293 cells transfected with 11beta-HSD1 | Homo sapiens |
K5S/K6S/E25K/E26K | 21% of wild type cortisone reduction activity | Homo sapiens |
K6S/E25K | 86% of wild type cortisone reduction activity | Homo sapiens |
K6S/E25K/E26K | 31% of wild type cortisone reduction activity | Homo sapiens |
K6S/E25K/E26Q | 30% of wild type cortisone reduction activity | Homo sapiens |
K6S/E25Q/E26Q | 43% of wild type cortisone reduction activity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00034 | - |
cortisol | 37°C, pH 7.4, oxidation of cortisol by lysates of HEK-293 cells transfected with 11beta-HSD1 | Homo sapiens | |
0.00034 | - |
cortisone | 37°C, pH 7.4, reduction of cortisone by lysates of HEK-293 cells transfected with 11beta-HSD1 | Homo sapiens | |
0.0008 | - |
cortisol | 37°C, pH 7.4, oxidation of cortisol in intact HEK-293 cells transfected with 11beta-HSD1 | Homo sapiens | |
0.0008 | - |
cortisone | 37°C, pH 7.4, reduction of cortisone in intact HEK-293 cells transfected with 11beta-HSD1 | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | - |
Homo sapiens | 5783 | - |
membrane | membrane bound, luminal orientation of 11beta-HSD1 is essential for efficient oxidation of cortisol | Homo sapiens | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cortisone + NADPH + H+ | Homo sapiens | - |
cortisol + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7-oxocholesterol + NADPH + H+ | luminal orientation of 11beta-HSD1 is essential for the reduction of 7-oxocholesterol | Homo sapiens | 7beta-hydroxycholesterol + NADP+ | - |
? | |
cortisone + NADPH + H+ | - |
Homo sapiens | cortisol + NADP+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
11beta-HSD1 | - |
Homo sapiens |
11beta-hydroxysteroid dehydrogenase | - |
Homo sapiens |