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Literature summary for 1.1.1.145 extracted from

  • Ohno, S.; Matsumoto, N.; Watanabe, M.; Nakajin, S.
    Flavonoid inhibition of overexpressed human 3beta-hydroxysteroid dehydrogenase type II (2004), J. Steroid Biochem. Mol. Biol., 88, 175-182.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Sf9 cells Homo sapiens
expression of the isozyme type II in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
6-hydroxyflavone 0.00056 mM, 50% inhibition; competitive Homo sapiens
apigenin 0.13 mM, 50% inhibition Homo sapiens
Biochanin A 0.00094 mM, 50% inhibition Homo sapiens
cyanoketone 0.0000076 mM, 50% inhibition; competitive Homo sapiens
daidzein 0.0033 mM, 50% inhibition Homo sapiens
flavanones diverse derivatives, inhibitory potential determination, overview Homo sapiens
flavones diverse derivatives, inhibitory potential determination, overview Homo sapiens
formononetin 0.0032 mM, 50% inhibition Homo sapiens
genistein 0.00026 mM, 50% inhibition; competitive Homo sapiens
isoflavones diverse derivatives, inhibitory potential determination, overview Homo sapiens
additional information structure-inhibition relationship Homo sapiens
myricetin 0.011 mM, 50% inhibition Homo sapiens
quercetin 0.084 mM, 50% inhibition Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0018
-
dehydroepiandrosterone 37°C, pH 7.4 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dehydroepiandrosterone + NAD+ Homo sapiens
-
androst-5-en-3,17-dione + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Reaction

Reaction Comment Organism Reaction ID
a 3beta-hydroxy-DELTA5-steroid + NAD+ = a 3-oxo-DELTA5-steroid + NADH + H+ structure-function relationship Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dehydroepiandrosterone + NAD+
-
Homo sapiens androst-5-en-3,17-dione + NADH + H+
-
?

Synonyms

Synonyms Comment Organism
3beta-HSD type II
-
Homo sapiens
3beta-hydroxysteroid dehydrogenase type II
-
Homo sapiens
3beta-hydroxysteroid dehydrogenase/DELTA5-DELTA4-isomerase type II
-
Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00000067
-
cyanoketone recombinant enzyme Homo sapiens
0.00000067
-
cyanoketone 37°C, pH 7.4 Homo sapiens
0.00012
-
genistein recombinant enzyme Homo sapiens
0.00012
-
genistein 37°C, pH 7.4 Homo sapiens
0.00019
-
6-hydroxyflavone recombinant enzyme Homo sapiens
0.00019
-
6-hydroxyflavone 37°C, pH 7.4 Homo sapiens