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Literature summary for 1.1.1.143 extracted from
- Enzymic epimerization of sequoyitol to D-pinitol in Trifolium incarnatum (1969), Eur. J. Biochem., 7, 442-448.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| DTT | 5fold activation | Trifolium incarnatum |  |
| GSH | 5fold activation | Trifolium incarnatum | |
| L-cysteine | 5fold activation at 1.3 mM | Trifolium incarnatum | |
General Stability
| General Stability | Organism |
|---|---|
| very unstable | Trifolium incarnatum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| iodoacetate | 85% inhibition at 1 mM | Trifolium incarnatum | |
| p-chloromercuribenzoate | complete inhibition at 1 mM | Trifolium incarnatum | |
| Phenylmercurinitrate | complete inhibition at 1 mM | Trifolium incarnatum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
gel filtration | Trifolium incarnatum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-O-methyl-myo-inositol + NAD+ | Trifolium incarnatum | B-specificity | 5D-5-O-methyl-2,3,5/4,6-pentahydroxycyclohexanone + NADH | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trifolium incarnatum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Trifolium incarnatum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| plant | prior to the formation of flowers | Trifolium incarnatum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,4,6/3,5-pentahydroxycyclohexanone + NADH | - |
Trifolium incarnatum | ? + NAD+ | - |
? | |
| 5-O-methyl-myo-inositol + NAD+ | B-specificity | Trifolium incarnatum | 5D-5-O-methyl-2,3,5/4,6-pentahydroxycyclohexanone + NADH | - |
r | |
| DL-2,3,4,6/5-pentahydroxycyclohexanone + NADH | low activity | Trifolium incarnatum | ? + NAD+ | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | - |
Trifolium incarnatum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Trifolium incarnatum | |
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Release 2023.1 (January 2023)
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