General Stability | Organism |
---|---|
NAD+ stabilizes | Homo sapiens |
no stabilization by addition of prostaglandins | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
13-cis-Prostaglandin F2alpha | 0.124 mM, 50% inhibition | Homo sapiens | |
15-epi-prostaglandin E1 | - |
Homo sapiens | |
15-Epiprostaglandin E1 | 0.17 mM, 50% inhibition | Homo sapiens | |
7-Oxa-13-prostynoic acid | 0.0475 mM, 50% inhibition | Homo sapiens | |
7-oxa-prostanoic acid | - |
Homo sapiens | |
7-Oxoprostanoic acid | - |
Homo sapiens | |
7-Thia-13-prostynoic acid | 0.0068 mM, 50% inhibition | Homo sapiens | |
7-thia-prostanoic acid | - |
Homo sapiens | |
arachidonic acid | 0.0027 mM, 50% inhibition; irreversible inactivation | Homo sapiens | |
ent-13-dehydro-15-epi-prostaglandin F2alpha | - |
Homo sapiens | |
Ent-13-dehydro-15-epiprostaglandin F3alpha | 0.055 mM, 50% inhibition | Homo sapiens | |
ent-13-dehydroprostaglandin E2 | 0.0068 mM, 50% inhibition | Homo sapiens | |
epi-7-thia-prostaglandin F2alpha | - |
Homo sapiens | |
epi-7-thiaprostaglandin F2alpha | 0.0052 mM, 50% inhibition | Homo sapiens | |
Ethacrynic acid | 0.0055 mM, 50% inhibition; irreversible inactivation | Homo sapiens | |
additional information | IC50 values at pH 7.0 and at pH 9.0; pH-dependent inhibition and inhibition mechanism, at high substrate concentrations there is formation of unreactive complexes between the 15-hydroxyrostaglandin and both the free enzyme and enzyme-NADH complex and between the 15-ketoprostaglandin and both the free enzyme and enzyme NAD+ complex | Homo sapiens | |
nat-13-dehydroprostaglandin E2 | - |
Homo sapiens | |
nat-13-dehydroprostaglandin F3alpha | - |
Homo sapiens | |
Nat-13-thiaprostaglandin F2alpha | 0.0088 mM, 50% inhibition | Homo sapiens | |
nat-7-thia-prostaglandin F2alpha | - |
Homo sapiens | |
nat-l3-dehydroprostaglandin F2alpha | - |
Homo sapiens | |
Prostaglandin B1 | 0.0497 mM, 50% inhibition | Homo sapiens | |
Prostanoic acid | 0.013 mM, 50% inhibition | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics, overview | Homo sapiens | |
0.0013 | - |
prostaglandin E1 | at pH 7.0 | Homo sapiens | |
0.0025 | - |
prostaglandin E2 | at pH 7.0 | Homo sapiens | |
0.0028 | - |
prostaglandin E2 | at pH 9.0 | Homo sapiens | |
0.0048 | - |
prostaglandin E1 | at pH 9.0 | Homo sapiens | |
0.0156 | - |
NADH | at pH 7.0, + 15-keto-PGE1 | Homo sapiens | |
0.0156 | - |
NADH | at pH 7.0, + 15-ketoprostaglandin E2 | Homo sapiens | |
0.0227 | - |
NAD+ | at pH 9.0, + prostaglandin E2 | Homo sapiens | |
0.0266 | - |
15-ketoprostaglandin E2 | at pH 9.0 | Homo sapiens | |
0.0284 | - |
NAD+ | at pH 7.0, + prostaglandin E2 | Homo sapiens | |
0.029 | - |
NAD+ | at pH 9.0, + prostaglandin E1 | Homo sapiens | |
0.0308 | - |
NAD+ | at pH 7.0, + prostaglandin E1 | Homo sapiens | |
0.0521 | - |
15-ketoprostaglandin E1 | at pH 7.0 | Homo sapiens | |
0.0528 | - |
15-ketoprostaglandin E2 | at pH 7.0 | Homo sapiens | |
0.0594 | - |
NADH | at pH 9.0, + 15-ketoprostaglandin E2 | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate + NAD+ | Homo sapiens | first step in prostaglnadin catabolism | (5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(5Z,13E,15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate + NAD+ = (5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NADH + H+ | single displacement mechanism | Homo sapiens | |
(5Z,13E,15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate + NAD+ = (5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NADH + H+ | displacement reaction mechanism, at pH 7.0 a kinetically significant ternary complex is formed, while at pH 9.0 the ternary complex is not kinetically significant, Theorell-Chance mechanism | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
placenta | - |
Homo sapiens | - |
Storage Stability | Organism |
---|---|
-20°C, purified enzyme in a buffer containing 50% glycerol, 5 mM potassium phosphate, pH 7.0, and 1 mM EDTA, loss less than 10% of its initial activity in 2 months | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate + NAD+ | first step in prostaglnadin catabolism | Homo sapiens | (5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NADH + H+ | - |
? | |
(5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate + NAD+ | i.e. 15-hydroxyprostaglandin or (15S)-PGE2 | Homo sapiens | (5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NADH + H+ | - |
? | |
prostaglandin E1 + NAD+ | - |
Homo sapiens | 15-ketoprostaglandin E1 + NADH + H+ | - |
? | |
prostaglandin E2 + NAD+ | - |
Homo sapiens | 15-ketoprostaglandin E2 + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
15-hydroxyprostaglandin dehydrogenase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | specific for | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics | Homo sapiens |