Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | gut genes are induced by sorbitol. GutR codes for a transcriptional activator of gut genes | Lacticaseibacillus casei |
Cloned (Comment) | Organism |
---|---|
His-tagged GutR and GutM expressed in Escherichia coli M15(pREP4) | Lacticaseibacillus casei |
Protein Variants | Comment | Organism |
---|---|---|
additional information | mutant with a frameshift in gutR (strain BL285). The mutant loses the ability to ferment sorbitol, and transcription of the gut operon in cells grown with a mixture of ribose plus sorbitol is abolished. Sorbitol uptake rate in strain BL285 is 88fold lower than in the wild-type. GutB mutant (strain BL282) constitutively expresses the gut operon but is impaired in sorbitol uptake. GutM mutant (strain BL286) shows lower levels of gutFRMCBA transcription than the wild-type strain | Lacticaseibacillus casei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | gut genes are repressed by glucose | Lacticaseibacillus casei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | GutM | Lacticaseibacillus casei | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lacticaseibacillus casei | - |
ATCC 334 and BL23 | - |
Purification (Comment) | Organism |
---|---|
GutR and GutM purified on Ni-NTA column | Lacticaseibacillus casei |
Storage Stability | Organism |
---|---|
-80°C, 10 mM NaH2PO4 buffer, pH 7.5, 45% glycerol, 10 mM beta-mercaptoethanol | Lacticaseibacillus casei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-sorbitol 6-phosphate + NAD+ | - |
Lacticaseibacillus casei | D-fructose 6-phosphate + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GutF | - |
Lacticaseibacillus casei |
GutM | - |
Lacticaseibacillus casei |
GutR | - |
Lacticaseibacillus casei |
operon gutFRMCBA | - |
Lacticaseibacillus casei |
sorbitol-6-phosphate dehydrogenase | - |
Lacticaseibacillus casei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Lacticaseibacillus casei |