Cloned (Comment) | Organism |
---|---|
cloning and subcloning of the gut operon and gutD gene | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diethyl dicarbonate | 100% inactivation | Escherichia coli | |
N-ethylmaleimide | about 90% inactivation | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
NAD+ | - |
Escherichia coli | |
3.3 | - |
D-sorbitol 6-phosphate | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
4 * 26000, SDS-PAGE | Escherichia coli |
117000 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli LJ378 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
180 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, more than 4 months, no appreciable loss of activity | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-sorbitol 6-phosphate + NAD+ | high specificity for NAD+ | Escherichia coli | D-fructose 6-phosphate + NADH + H+ | - |
? | |
D-sorbitol 6-phosphate + NAD+ | high specificity for D-sorbitol 6-phosphate | Escherichia coli | D-fructose 6-phosphate + NADH + H+ | - |
? | |
D-sorbitol 6-phosphate + NAD+ | high specificity for NAD+ | Escherichia coli LJ378 | D-fructose 6-phosphate + NADH + H+ | - |
? | |
D-sorbitol 6-phosphate + NAD+ | high specificity for D-sorbitol 6-phosphate | Escherichia coli LJ378 | D-fructose 6-phosphate + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 26000, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
assay at room temperature | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
20 min, 85% loss of activity | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.7 | - |
- |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with NADP+ | Escherichia coli | |
NAD+ | specific for | Escherichia coli |