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Literature summary for 1.1.1.138 extracted from
- Purification and properties of mannitol dehydrogenase from Agaricus bisporus sporocarps (1978), Phytochemistry, 17, 865-868.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | inhibition of fructose reduction, NADP+/NADPH ratio of 4:1 necessary for half maximal activity | Agaricus bisporus |  |
| NADPH | inhibition of D-mannitol oxidation, NADP+/NADPH ratio of 0.25 necessary for half maximal activity | Agaricus bisporus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00525 | - |
NADPH | - |
Agaricus bisporus | |
| 34 | - |
D-mannitol | - |
Agaricus bisporus | |
| 190 | - |
D-fructose | - |
Agaricus bisporus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | 37% activation at 5 mM only in D-mannitol oxidation | Agaricus bisporus | |
| Ca2+ | 52% activation at 5 mM up to 140% at 10 mM only in D-mannitol oxidation | Agaricus bisporus | |
| Mg2+ | 36% activation at 5 mM only in D-mannitol oxidation | Agaricus bisporus | |
| Mn2+ | 15% activation at 5 mM only in D-mannitol oxidation | Agaricus bisporus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
gel filtration | Agaricus bisporus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose + NADPH | Agaricus bisporus | - |
D-mannitol + NADP+ | - |
r | |
| D-mannitol + NADP+ | Agaricus bisporus | - |
D-fructose + NADPH + H+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Agaricus bisporus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, affinity chromatography | Agaricus bisporus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| sporocarp | - |
Agaricus bisporus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 148.6 | - |
- |
Agaricus bisporus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-arabinitol + NADP+ | 3% of the activity compared to D-mannitol | Agaricus bisporus | D-xylulose + NADPH + H+ | - |
? | |
| D-fructose + NADPH | - |
Agaricus bisporus | D-mannitol + NADP+ | - |
r | |
| D-mannitol + NADP+ | - |
Agaricus bisporus | D-fructose + NADPH + H+ | - |
r | |
| D-sorbitol + NADP+ | 21% of the activity compared to D-mannitol | Agaricus bisporus | D-sorbose + NADPH + H+ | - |
? | |
| L-arabinitol + NADP+ | 2% of the activity compared to D-mannitol | Agaricus bisporus | L-xylulose + NADPH + H+ | - |
? | |
| perseitol + NADP+ | 6% of the activity compared to D-mannitol | Agaricus bisporus | ? + NADPH | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 47 | - |
- |
Agaricus bisporus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.9 | 8.1 | D-fructose reduction | Agaricus bisporus |
| 8.7 | 8.8 | D-mannitol oxidation | Agaricus bisporus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | specific for NADP+ | Agaricus bisporus | |
| NADPH | cannot be replaced by NADH | Agaricus bisporus | |
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