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Literature summary for 1.1.1.133 extracted from

  • van der Beek, S.L.; Le Breton, Y.; Ferenbach, A.T.; Chapman, R.N.; van Aalten, D.M.; Navratilova, I.; Boons, G.J.; McIver, K.S.; van Sorge, N.M.; Dorfmueller, H.C.
    GacA is essential for group A Streptococcus and defines a new class of monomeric dTDP-4-dehydrorhamnose reductases (RmlD) (2015), Mol. Microbiol., 98, 946-962.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Streptococcus sp.

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 12.5% (w/v) PEG 1000, 12.5% (w/v) PEG 3350, 12.5% (v/v) 2-methyl-2,4-pentanediol, 0.02 M of each carboxylic acid (0.2 M sodium formate, 0.2 M ammonium acetate, 0.2 M trisodium citrate, 0.2 M sodium potassium L-tartrate, 0.2 M sodium oxamate) and 0.1 M MES/imidazole pH 6.5 Streptococcus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.37
-
 dTDP-alpha-D-glucose at pH 7.5, temperature not specified in the publication Streptococcus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme does not require metal for its activity Streptococcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27500
-
 1 * 27500, SDS-PAGE Streptococcus sp.
32000
-
-
 Streptococcus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dTDP-beta-L-rhamnose + NADP+ Streptococcus sp.
-
 dTDP-4-dehydro-beta-L-rhamnose + NADPH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Streptococcus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and Superdex 75 gel filtration Streptococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dTDP-alpha-D-glucose + NADP+
-
 Streptococcus sp. ?
-
 ?
dTDP-beta-L-rhamnose + NADP+
-
 Streptococcus sp. dTDP-4-dehydro-beta-L-rhamnose + NADPH + H+
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 27500, SDS-PAGE Streptococcus sp.
monomer 1 * 32000, calculated from amino acid sequence Streptococcus sp.

Synonyms

Synonyms Comment Organism
dTDP-4-dehydrorhamnose reductase
-
 Streptococcus sp.
gacA
-
 Streptococcus sp.
RmlD
-
 Streptococcus sp.

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Streptococcus sp.