Cloned (Comment) | Organism |
---|---|
- |
Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibitory: MgCl2, CaCl2, EDTA at 10mM | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
NADPH | pH 8.5, 30°C | Arabidopsis thaliana | |
0.0169 | - |
dTDP-4-keto-6-deoxy-D-glucose | pH 8.5, 30°C | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
x * 35500, deduced from gene sequence, x * 35000, SDS-PAGE | Arabidopsis thaliana |
35500 | - |
x * 35500, deduced from gene sequence, x * 35000, SDS-PAGE | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9LQ04 | bifunctional 3,5-epimerase/4-keto reductase | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Arabidopsis thaliana | - |
leaf | - |
Arabidopsis thaliana | - |
additional information | present in all tissues examined | Arabidopsis thaliana | - |
root | - |
Arabidopsis thaliana | - |
Storage Stability | Organism |
---|---|
-20°C, repeated freezing and thawing, loss of more than 60% of activity | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTDP-4-dehydro-6-deoxy-D-glucose + NADH + H+ | 40% of the rate with NADPH | Arabidopsis thaliana | dTDP-beta-L-rhamnose + NAD+ | - |
? | |
dTDP-4-dehydro-6-deoxy-D-glucose + NADPH | - |
Arabidopsis thaliana | dTDP-beta-L-rhamnose + NADP+ | - |
? | |
additional information | no substrate: GDP-4-keto-6-deoxy-mannose, UDP-glucose, dTDP-glucose, UDP-xylose, GDP-fucose | Arabidopsis thaliana | ? | - |
? | |
UDP-4-dehydro-6-deoxy-D-glucose + NADPH | - |
Arabidopsis thaliana | UDP-beta-L-rhamnose + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 35500, deduced from gene sequence, x * 35000, SDS-PAGE | Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Arabidopsis thaliana |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
about 35% of maximum activity | Arabidopsis thaliana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7.5 | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Arabidopsis thaliana | |
NADPH | - |
Arabidopsis thaliana |