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Literature summary for 1.1.1.133 extracted from
- The mechanism of 6-deoxyhexose synthesis. II. Conversion of deoxythymidine diphosphate 4-keto-6-deoxy-D-glucose to deoxythymidine diphosphate L-rhamnose (1968), J. Biol. Chem., 243, 1475-1478.
General Stability
| General Stability | Organism |
|---|---|
| NADPH and to a lesser degree NADP+ prevent denaturation at 50°C, NAD+ and NADH are less effective | Pseudomonas aeruginosa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
ATCC 7700 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, affinity chromatography, co-purification of enzyme I and II | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dTDP-4-dehydro-6-deoxy-D-glucose + NADPH + H+ | - |
Pseudomonas aeruginosa | dTDP-L-rhamnose + NADP+ | - |
? |  |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
NADPH more than NADP+ prevents heat denaturation during 5 and 8 min | Pseudomonas aeruginosa |
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