Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5'-(3-hydroxypropyl)-amino-5'-deoxy-guanosine | only after preincubation with enzyme | Pseudomonas aeruginosa | |
5'-azido-5'-deoxy-guanosine | only after preincubation with enzyme | Pseudomonas aeruginosa | |
5'-azido-5'-deoxy-N2-2',3'-O-triacetylguanosine | only after preincubation with enzyme | Pseudomonas aeruginosa | |
5'[[[[(2'',3'',4'',6''-tetra-O-acetyl 6''-ethynyl)alpha-D-mannopyranosyl oxy]-carbonyl]amino]sulfonyl]-2',3'-isopropylidene N2-acetyl guanosine | GDP-analog, 0.1 mM, 60% inhibition | Pseudomonas aeruginosa | |
guanosine | slight inhibition only after preincubation with enzyme | Pseudomonas aeruginosa | |
additional information | strains which resist 0.005 mg/ml of tobramycin treated by 5'-(3-hydroxypropyl)-amino-5'-deoxy-guanosine, tetra-O-acetylmannopyranose and 5'[[[[(2'',3'',4'',6''-tetra-O-acetyl 6''-ethynyl)alpha-D-mannopyranosyl oxy]-carbonyl]amino]sulfonyl]-2',3'-isopropylidene N2-acetyl guanosine recover 20, 75 and 85% of their sensisivity towards tobramycin | Pseudomonas aeruginosa | |
N2-acetyl-2',3'-isopropylidene guanosine | only after preincubation with enzyme | Pseudomonas aeruginosa | |
N2-acetyl-5'-amino-5'-deoxy-2',3'-O-acetylguanosine | only after preincubation with enzyme | Pseudomonas aeruginosa | |
N2-acetyl-5'-amino-5'-deoxy-guanosine | only after preincubation with enzyme | Pseudomonas aeruginosa | |
N2-acetyl-guanosine | only after preincubation with enzyme | Pseudomonas aeruginosa | |
tetra-O-acetylmannopyranose | GDP-analog, 0.1 mM, 75% inhibition | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
A22 alg+, selected from mucoid strain IPA22 | - |
Purification (Comment) | Organism |
---|---|
protamine sulfate precipitation, gel exclusion, ion exchange chromatography | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-mannose + NAD+ + H2O | - |
Pseudomonas aeruginosa | GDP-mannuronate + NADH | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas aeruginosa |