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Literature summary for 1.1.1.120 extracted from

  • Schiwara, H.W.; Domagk, G.F.
    Anreicherung und Charakterisierung einer NADP+-abhängigen Abequosedehydrogenase aus Pseudomonas putida (1968), Hoppe-Seyler's Z. Physiol. Chem., 349, 1321-1329.
    View publication on PubMed
Search for more literature for 1.1.1.120

General Stability

General Stability Organism
very stable at -15°C Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
cysteine 82% inhibition at 20 mM Pseudomonas putida
glutathione 27% inhibition at 20 mM Pseudomonas putida
Hg2+ complete inhibition at 1 mM Pseudomonas putida
p-chloromercuribenzoate complete inhibition at 1 mM, partial reversible by low concentration of cysteine Pseudomonas putida
thioglycolate 96% inhibition at 10 mM Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.037
-
 NADP+
-
 Pseudomonas putida
3.3
-
 3,6-Dideoxy-D-galactose
-
 Pseudomonas putida
12
-
 2-deoxy-D-galactose
-
 Pseudomonas putida
48
-
 3-Deoxy-D-galactose
-
 Pseudomonas putida
60
-
 6-deoxy-D-galactose
-
 Pseudomonas putida
130
-
 2,6-dideoxy-D-allose
-
 Pseudomonas putida
420
-
 D-galactose
-
 Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-galactose + NADP+ Pseudomonas putida furanose form, beta-configuration D-galactonolactone + NADPH gamma-lactone ?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Pseudomonas putida

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Pseudomonas putida
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.88
-
 substrate: D-galactose Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,6-dideoxy-D-allofuranose + NADP+ i.e. digitoxose, furanose form, 81% of the activity compared to abequose Pseudomonas putida ? + NADPH
-
 ?
2,6-dideoxy-D-allose + NADP+ 81% activity compared to 3,6-dideoxy-D-galactose Pseudomonas putida ?
-
 r
2-deoxy-D-galactose + NADP+ 114% of the activity compared to abequose Pseudomonas putida D-galactonolactone + NADPH
-
 ?
2-deoxy-D-ribose + NADP+ 18% of the activity compared to abequose Pseudomonas putida ? + NADPH
-
 ?
3,6-dideoxy-beta-D-galactofuranose + NADP+ i.e. abequose, furanose form, beta-configuration Pseudomonas putida 3,6-dideoxy-D-galactono-1,4-lactone + NADPH
-
 ?
3,6-dideoxy-D-galactose + NADP+ 100% activity Pseudomonas putida ?
-
 r
3-deoxy-D-galactose + NADP+ 36% of the activity compared to abequose Pseudomonas putida D-galactonolactone + NADPH
-
 ?
6-deoxy-D-galactose + NADP+ 94% of the activity compared to abequose Pseudomonas putida D-galactonolactone + NADPH
-
 ?
beta-D-galactofuranose + NADP+ furanose form, beta-configuration Pseudomonas putida D-galactono-1,4-lactone + NADPH
-
 ?
D-arabinose + NADP+ 5% of the activity compared to abequose Pseudomonas putida D-arabinolactone + NADPH
-
 ?
D-galactose + NADP+ furanose form, beta-configuration Pseudomonas putida D-galactonolactone + NADPH gamma-lactone ?
D-ribose + NADP+ traces of activity Pseudomonas putida ? + NADPH
-
 ?
L-arabinose + NADP+ 6% of the compared to abequose Pseudomonas putida L-arabinolactone + NADPH
-
 ?

Synonyms

Synonyms Comment Organism
abequosedehydrogenase
-
 Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10.5
-
 substrate: abequose Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
NADP+ cannot be replaced by NAD+ Pseudomonas putida