Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.119 extracted from

  • Bonete, M.J.; Pire, C.; Llorca, F.I.; Camacho, M.L.
    Glucose dehydrogenase from the halophilic Archaeon Haloferax mediterranei: Enzyme purification, characterization and N-terminal sequence (1996), FEBS Lett., 383, 227-229.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
EDTA 2 mM Haloferax mediterranei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.024
-
NADP+ pH 8.8, 40°C Haloferax mediterranei
1.2
-
NAD+ pH 8.8, 40°C Haloferax mediterranei
3.9
-
beta-D-glucose pH 8.8, 40°C Haloferax mediterranei

Metals/Ions

Metals/Ions Comment Organism Structure
KCl the activity is dependent on the concentration of NaCl or KCl in the activity buffer, being optimal at 1.3 M Haloferax mediterranei
Mg2+ besides KCl/NaCl, the activity also depends on presence of bivalent cations. Mg2+ is more effective than Mg2+ or Ni2+ Haloferax mediterranei
Mn2+ besides KCl/NaCl, the activity also depends on presence of bivalent cations. Mn2+ is less effective than Mg2+ and more effective than Ni2+ Haloferax mediterranei
NaCl the activity is dependent on the concentration of NaCl or KCl in the activity buffer, being optimal at 1.3 M Haloferax mediterranei
Ni2+ besides KCl/NaCl, the activity also depends on presence of bivalent cations. Ni2+ is more effective than Mg2+ or Mn2+ Haloferax mediterranei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
2 * 53000, SDS-PAGE Haloferax mediterranei
89000
-
gel filtration Haloferax mediterranei

Organism

Organism UniProt Comment Textmining
Haloferax mediterranei Q977U7
-
-
Haloferax mediterranei DSM 1411 Q977U7
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Haloferax mediterranei

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
550
-
pH 8.8, 40°C Haloferax mediterranei

Storage Stability

Storage Stability Organism
4°C, stable for several months Haloferax mediterranei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-glucose + NAD+ activity with NAD+ is 26% of the activity with NADP+ Haloferax mediterranei ?
-
?
beta-D-glucose + NAD+ activity with NAD+ is 26% of the activity with NADP+ Haloferax mediterranei DSM 1411 ?
-
?
beta-D-glucose + NADP+ activity with NAD+ is 26% of the activity with NADP+. The enzyme shows also activity with D-fucose and NADP+ (at 18% compared to the activity with beta-D-glucose and NADP+), with D-galactose and NADP+ (at 13% compared to the activity with beta-D-glucose and NADP+), with D-xylose and NAD+ (at 79% compared to the activity with beta-D-glucose and NADP+), with D-xylose and NADP+ (at 88% compared to the activity with beta-D-glucose and NADP+) Haloferax mediterranei ?
-
?
beta-D-glucose + NADP+ activity with NAD+ is 26% of the activity with NADP+. The enzyme shows also activity with D-fucose and NADP+ (at 18% compared to the activity with beta-D-glucose and NADP+), with D-galactose and NADP+ (at 13% compared to the activity with beta-D-glucose and NADP+), with D-xylose and NAD+ (at 79% compared to the activity with beta-D-glucose and NADP+), with D-xylose and NADP+ (at 88% compared to the activity with beta-D-glucose and NADP+) Haloferax mediterranei DSM 1411 ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 53000, SDS-PAGE Haloferax mediterranei

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
half-life: 56.8 h, in the presence of 3 M NaCl, stability decreases significantly with lower salt concentrations Haloferax mediterranei

Cofactor

Cofactor Comment Organism Structure
NAD+ Km-value for NAD+ is 50fold higher than Km-value for NADP+ Haloferax mediterranei
NADP+ Km-value for NAD+ is 50fold higher than Km-value for NADP+ Haloferax mediterranei