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Literature summary for 1.1.1.119 extracted from

  • Adachi, O.; Matsushita, K.; Shinagawa, E.; Ameyama, M.
    Crystallization and characterization of NADP-dependent D-glucose dehydrogenase from Gluconobacter suboxydans (1980), Agric. Biol. Chem., 44, 301-308.
No PubMed abstract available

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Gluconobacter oxydans

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
Gluconobacter oxydans
Hg2+
-
Gluconobacter oxydans
Ni2+
-
Gluconobacter oxydans
p-chloromercuribenzoate
-
Gluconobacter oxydans
sulfhydryl reagents
-
Gluconobacter oxydans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
NADP+
-
Gluconobacter oxydans
5
-
D-glucose
-
Gluconobacter oxydans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
4 * 40000, SDS-PAGE Gluconobacter oxydans
153000
-
gel filtration on Sephadex G-200 Gluconobacter oxydans

Organism

Organism UniProt Comment Textmining
Gluconobacter oxydans
-
IFO 12528
-

Purification (Commentary)

Purification (Comment) Organism
column chromatography on DEAE-Sephadex A-50 and affinty chromatography by blue-dextran Sepharose 4B Gluconobacter oxydans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
140
-
-
Gluconobacter oxydans

Storage Stability

Storage Stability Organism
5°C, crystalline preparation in ammonium sulfate, 6 months Gluconobacter oxydans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose + NADP+ 4% of the reverse reaction when assayed with D-glucono-1,5-lactone and NADPH at neutral or weak alkaline pH Gluconobacter oxydans D-glucono-1,5-lactone + NADPH + H+
-
r
D-mannose + NADP+
-
Gluconobacter oxydans D-mannono-1,5-lactone + NADPH
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 40000, SDS-PAGE Gluconobacter oxydans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Gluconobacter oxydans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 9 in Tris-buffer Gluconobacter oxydans

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Gluconobacter oxydans
NADPH
-
Gluconobacter oxydans