Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JM109 cells | Xanthomonas translucens |
Protein Variants | Comment | Organism |
---|---|---|
D298A | inactive | Xanthomonas translucens |
E185A | the mutant shows reduced activity compared to the wild type enzyme | Xanthomonas translucens |
E196A | inactive | Xanthomonas translucens |
E201A | the mutant shows reduced activity compared to the wild type enzyme | Xanthomonas translucens |
F189A | the mutant shows reduced activity compared to the wild type enzyme | Xanthomonas translucens |
F234A | the mutant shows reduced activity compared to the wild type enzyme | Xanthomonas translucens |
F249A | inactive | Xanthomonas translucens |
F253A | inactive | Xanthomonas translucens |
M231A | the mutant shows reduced activity compared to the wild type enzyme | Xanthomonas translucens |
M246A | the mutant shows reduced activity compared to the wild type enzyme | Xanthomonas translucens |
N144A | the mutant shows reduced activity compared to the wild type enzyme | Xanthomonas translucens |
Q252A | the mutant shows reduced activity compared to the wild type enzyme | Xanthomonas translucens |
R193A | inactive | Xanthomonas translucens |
R200A | the mutant shows reduced activity compared to the wild type enzyme | Xanthomonas translucens |
R297A | the mutant shows reduced activity compared to the wild type enzyme | Xanthomonas translucens |
S117A | the mutant shows reduced activity compared to the wild type enzyme | Xanthomonas translucens |
S120A | the mutant shows reduced activity compared to the wild type enzyme | Xanthomonas translucens |
T262A | the mutant shows reduced activity compared to the wild type enzyme | Xanthomonas translucens |
W199A | inactive | Xanthomonas translucens |
W228A | the mutant shows reduced activity compared to the wild type enzyme | Xanthomonas translucens |
W261A | inactive | Xanthomonas translucens |
Y147A | the mutant shows reduced activity compared to the wild type enzyme | Xanthomonas translucens |
Y237A | the mutant shows reduced activity compared to the wild type enzyme | Xanthomonas translucens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
NAD+ | mutant enzyme W228A, at pH 9.5, temperature not specified in the publication | Xanthomonas translucens | |
0.08 | - |
NAD+ | mutant enzyme Y147A, at pH 9.5, temperature not specified in the publication | Xanthomonas translucens | |
0.09 | - |
NAD+ | mutant enzyme F234A, at pH 9.5, temperature not specified in the publication | Xanthomonas translucens | |
0.09 | - |
NAD+ | mutant enzyme M231A, at pH 9.5, temperature not specified in the publication | Xanthomonas translucens | |
0.26 | - |
NAD+ | mutant enzyme R297A, at pH 9.5, temperature not specified in the publication | Xanthomonas translucens | |
0.31 | - |
NAD+ | wild type enzyme, at pH 9.5, temperature not specified in the publication | Xanthomonas translucens | |
0.35 | - |
NAD+ | mutant enzyme N144A, at pH 9.5, temperature not specified in the publication | Xanthomonas translucens | |
0.35 | - |
NAD+ | mutant enzyme Y147A, at pH 9.5, temperature not specified in the publication | Xanthomonas translucens | |
0.36 | - |
NAD+ | mutant enzyme E185A, at pH 9.5, temperature not specified in the publication | Xanthomonas translucens | |
0.39 | - |
NAD+ | mutant enzyme M246A, at pH 9.5, temperature not specified in the publication | Xanthomonas translucens | |
0.39 | - |
NAD+ | mutant enzyme Y237A, at pH 9.5, temperature not specified in the publication | Xanthomonas translucens | |
0.4 | - |
NAD+ | mutant enzyme Q252A, at pH 9.5, temperature not specified in the publication | Xanthomonas translucens | |
0.53 | - |
NAD+ | mutant enzyme T262A, at pH 9.5, temperature not specified in the publication | Xanthomonas translucens | |
1.04 | - |
NAD+ | mutant enzyme F189A, at pH 9.5, temperature not specified in the publication | Xanthomonas translucens | |
2.25 | - |
NAD+ | mutant enzyme R200A, at pH 9.5, temperature not specified in the publication | Xanthomonas translucens | |
3.76 | - |
NAD+ | mutant enzyme S117A, at pH 9.5, temperature not specified in the publication | Xanthomonas translucens | |
9.66 | - |
NAD+ | mutant enzyme S120A, at pH 9.5, temperature not specified in the publication | Xanthomonas translucens | |
10.2 | - |
carnitine | wild type enzyme, at pH 9.5, temperature not specified in the publication | Xanthomonas translucens | |
40.4 | - |
carnitine | mutant enzyme E185A, at pH 9.5, temperature not specified in the publication | Xanthomonas translucens | |
89.1 | - |
carnitine | mutant enzyme T262A, at pH 9.5, temperature not specified in the publication | Xanthomonas translucens | |
101.4 | - |
carnitine | mutant enzyme Q252A, at pH 9.5, temperature not specified in the publication | Xanthomonas translucens | |
164 | - |
carnitine | mutant enzyme N144A, at pH 9.5, temperature not specified in the publication | Xanthomonas translucens | |
205.7 | - |
carnitine | mutant enzyme F189A, at pH 9.5, temperature not specified in the publication | Xanthomonas translucens | |
284 | - |
carnitine | mutant enzyme Y147A, at pH 9.5, temperature not specified in the publication | Xanthomonas translucens | |
307.6 | - |
carnitine | mutant enzyme S117A, at pH 9.5, temperature not specified in the publication | Xanthomonas translucens | |
308.8 | - |
carnitine | mutant enzyme M231A, at pH 9.5, temperature not specified in the publication | Xanthomonas translucens | |
331.9 | - |
carnitine | mutant enzyme Y147A, at pH 9.5, temperature not specified in the publication | Xanthomonas translucens | |
336.2 | - |
carnitine | mutant enzyme M246A, at pH 9.5, temperature not specified in the publication | Xanthomonas translucens | |
667 | - |
carnitine | mutant enzyme S120A, at pH 9.5, temperature not specified in the publication | Xanthomonas translucens | |
767.1 | - |
carnitine | mutant enzyme R200A, at pH 9.5, temperature not specified in the publication | Xanthomonas translucens | |
799.7 | - |
carnitine | mutant enzyme Y237A, at pH 9.5, temperature not specified in the publication | Xanthomonas translucens | |
830.7 | - |
carnitine | mutant enzyme R297A, at pH 9.5, temperature not specified in the publication | Xanthomonas translucens | |
1010 | - |
carnitine | mutant enzyme W228A, at pH 9.5, temperature not specified in the publication | Xanthomonas translucens | |
1056 | - |
carnitine | mutant enzyme F234A, at pH 9.5, temperature not specified in the publication | Xanthomonas translucens | |
2000 | - |
carnitine | Km above 2000 mM, mutant enzyme D298A, at pH 9.5, temperature not specified in the publication | Xanthomonas translucens | |
2000 | - |
carnitine | Km above 2000 mM, mutant enzyme F249A, at pH 9.5, temperature not specified in the publication | Xanthomonas translucens | |
2000 | - |
carnitine | Km above 2000 mM, mutant enzyme F253A, at pH 9.5, temperature not specified in the publication | Xanthomonas translucens | |
2000 | - |
carnitine | Km above 2000 mM, mutant enzyme W261A, at pH 9.5, temperature not specified in the publication | Xanthomonas translucens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
carnitine + NAD+ | Xanthomonas translucens | - |
3-dehydrocarnitine + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xanthomonas translucens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
amylose resin column chromatography, and Superdex 200 gel filtration | Xanthomonas translucens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carnitine + NAD+ | - |
Xanthomonas translucens | 3-dehydrocarnitine + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Cdh | - |
Xanthomonas translucens |
L-carnitine dehydrogenase | - |
Xanthomonas translucens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
carnitine | mutant enzyme F234A, at pH 9.5, temperature not specified in the publication | Xanthomonas translucens | |
0.001 | - |
carnitine | mutant enzyme R200A, at pH 9.5, temperature not specified in the publication | Xanthomonas translucens | |
0.001 | - |
carnitine | mutant enzyme S120A, at pH 9.5, temperature not specified in the publication | Xanthomonas translucens | |
0.003 | - |
carnitine | mutant enzyme Y147A, at pH 9.5, temperature not specified in the publication | Xanthomonas translucens | |
0.007 | - |
carnitine | mutant enzyme Y147A, at pH 9.5, temperature not specified in the publication | Xanthomonas translucens | |
0.008 | - |
carnitine | mutant enzyme W228A, at pH 9.5, temperature not specified in the publication | Xanthomonas translucens | |
0.009 | - |
carnitine | mutant enzyme F189A, at pH 9.5, temperature not specified in the publication | Xanthomonas translucens | |
0.01 | - |
carnitine | mutant enzyme M231A, at pH 9.5, temperature not specified in the publication | Xanthomonas translucens | |
0.013 | - |
carnitine | mutant enzyme Y237A, at pH 9.5, temperature not specified in the publication | Xanthomonas translucens | |
0.016 | - |
carnitine | mutant enzyme R297A, at pH 9.5, temperature not specified in the publication | Xanthomonas translucens | |
0.019 | - |
carnitine | mutant enzyme S117A, at pH 9.5, temperature not specified in the publication | Xanthomonas translucens | |
0.044 | - |
carnitine | mutant enzyme M246A, at pH 9.5, temperature not specified in the publication | Xanthomonas translucens | |
0.068 | - |
carnitine | mutant enzyme T262A, at pH 9.5, temperature not specified in the publication | Xanthomonas translucens | |
0.079 | - |
carnitine | mutant enzyme N144A, at pH 9.5, temperature not specified in the publication | Xanthomonas translucens | |
0.157 | - |
carnitine | mutant enzyme Q252A, at pH 9.5, temperature not specified in the publication | Xanthomonas translucens | |
0.22 | - |
carnitine | mutant enzyme E185A, at pH 9.5, temperature not specified in the publication | Xanthomonas translucens | |
1.279 | - |
carnitine | wild type enzyme, at pH 9.5, temperature not specified in the publication | Xanthomonas translucens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Xanthomonas translucens |