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Literature summary for 1.1.1.107 extracted from

  • Tamura, A.; Yoshikane, Y.; Yokochi, N.; Yagi, T.
    Synthesis of 4-pyridoxolactone from pyridoxine using a combination of transformed Escherichia coli cells (2008), J. Biosci. Bioeng., 106, 460-465.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed from pET21a vector in Escherichia coli BL21(DE3) Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli JM109
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
13.7
-
Escherichia coli BL21(DE3) cells transformed with pET21a-adh-pld Escherichia coli
47.6
-
Escherichia coli BL21(DE3) cells transformed with pET21a-pld Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyridoxal + NAD+ cells coexpressing pyridoxal 4-dehydrogenase and alcohol dehydrogenase show a higher conversion rate than those expressing pyridoxal 4-dehydrogenase alone Escherichia coli 4-pyridoxolactone + NADH + H+
-
?
pyridoxal + NAD+ cells coexpressing pyridoxal 4-dehydrogenase and alcohol dehydrogenase show a higher conversion rate than those expressing pyridoxal 4-dehydrogenase alone Escherichia coli JM109 4-pyridoxolactone + NADH + H+
-
?

Synonyms

Synonyms Comment Organism
PLDH
-
Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Escherichia coli