Inhibitors | Comment | Organism | Structure |
---|---|---|---|
8-hydroxyquinoline | 1 mM, 78% inhibition | Microbacterium luteolum | |
o-phenanthroline | 1 mM, 15% inhibition | Microbacterium luteolum | |
pyridoxal | substrate inhibition, 90% inhibition at 2 mM | Microbacterium luteolum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.133 | - |
NAD+ | 30°C, pH 8.0 | Microbacterium luteolum | |
0.473 | - |
pyridoxal | 30°C, pH 8.0 | Microbacterium luteolum | |
1.41 | - |
NADP+ | 30°C, pH 8.0 | Microbacterium luteolum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
2 * 38000, SDS-PAGE | Microbacterium luteolum |
80000 | - |
gel filtration | Microbacterium luteolum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Microbacterium luteolum | - |
growth on pyridoxine as carbon and nitrogen source | - |
Purification (Comment) | Organism |
---|---|
- |
Microbacterium luteolum |
Storage Stability | Organism |
---|---|
4°C, 1% 2-mercaptoethanol, 24 h, no loss of activity, 2-mercaptoethanol is essential for stability | Microbacterium luteolum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no substrate: pyridoxal-5-phosphate, pyridoxamine, pyridoxine, 2-nitrobenzaldehyde, 3-nitrobenzaldehyde, 4-nitrobenzaldehyde, o-phthalaldehyde, 5-nitrosalicylaldehyde, 2-carboxybenzaldehyde | Microbacterium luteolum | ? | - |
? | |
pyridine-4-aldehyde + NAD+ | 0.9% of the activity against pyridoxal | Microbacterium luteolum | 4-pyridoxolactone + NADH + H+ | - |
? | |
pyridoxal + NAD+ | - |
Microbacterium luteolum | 4-pyridoxolactone + NADH + H+ | - |
? | |
pyridoxal + NADP+ | - |
Microbacterium luteolum | 4-pyridoxolactone + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 38000, SDS-PAGE | Microbacterium luteolum |
More | N-terminal amino acid analysis | Microbacterium luteolum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Microbacterium luteolum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
10 min, no residual activity | Microbacterium luteolum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | sodium phosphate buffer | Microbacterium luteolum |
8.5 | - |
MOPS buffer | Microbacterium luteolum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Microbacterium luteolum | |
NADP+ | - |
Microbacterium luteolum |