Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Cupriavidus necator |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 20% (w/v) PEG 3350 and 0.2 M CaCl2 | Cupriavidus necator |
Protein Variants | Comment | Organism |
---|---|---|
G184A | the catalytic efficiency of the mutant is 330fold lower than that of the wild type enzyme | Cupriavidus necator |
L80G | the catalytic efficiency of the mutant is 3300fold lower than that of the wild type enzyme | Cupriavidus necator |
T186N | the catalytic efficiency of the mutant is 330fold lower than that of the wild type enzyme | Cupriavidus necator |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.097 | - |
NAD+ | mutant enzyme T186N, at pH 10.0 and 30°C | Cupriavidus necator | |
0.115 | - |
NAD+ | mutant enzyme L80G, at pH 10.0 and 30°C | Cupriavidus necator | |
0.12 | - |
NAD+ | wild type enzyme, at pH 10.0 and 30°C | Cupriavidus necator | |
0.156 | - |
NAD+ | mutant enzyme G184A, at pH 10.0 and 30°C | Cupriavidus necator | |
15.2 | - |
L-threonine | wild type enzyme, at pH 10.0 and 30°C | Cupriavidus necator |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-threonine + NAD+ | Cupriavidus necator | - |
(2S)-2-amino-3-oxobutanoate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cupriavidus necator | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-Sepharose column chromatography and Superdex 75 gel filtration | Cupriavidus necator |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-threonine + NAD+ | - |
Cupriavidus necator | (2S)-2-amino-3-oxobutanoate + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
L-ThrDH | - |
Cupriavidus necator |
L-threonine dehydrogenase | - |
Cupriavidus necator |
ThrDH | - |
Cupriavidus necator |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
50 | - |
L-threonine | wild type enzyme, at pH 10.0 and 30°C | Cupriavidus necator |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Cupriavidus necator |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
NAD+ | mutant enzyme L80G, at pH 10.0 and 30°C | Cupriavidus necator | |
0.01 | - |
NAD+ | mutant enzyme G184A, at pH 10.0 and 30°C | Cupriavidus necator | |
0.01 | - |
NAD+ | mutant enzyme T186N, at pH 10.0 and 30°C | Cupriavidus necator | |
3.3 | - |
L-threonine | wild type enzyme, at pH 10.0 and 30°C | Cupriavidus necator |