Cloned (Comment) | Organism |
---|---|
ligated into the pTZ57R/T cloning vector. The recombinant pTZ-tdh plasmid digested with NcoI and EcoRI and the TDH gene ligated with pET-8c, giving the recombinant pET-tdh plasmid, used to transform Escherichia coli DH5alpha competent cells. Recombinant and purified pET-tdh plasmid expressed in Escherichia coli BL21 (DE3) | Thermococcus kodakarensis |
Crystallization (Comment) | Organism |
---|---|
by hanging-drop vapour-diffusion method, to 2.6 A resolution. Crystals grow in the tetragonal space group P43212, with unit-cell parameters a=b=124.5, c=271.1 A. There are four molecules in the asymmetric unit of the crystal | Thermococcus kodakarensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38016 | - |
4 * 38016, electrospray mass spectrometry and gel filtration | Thermococcus kodakarensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus kodakarensis | Q5JI69 | - |
- |
Purification (Comment) | Organism |
---|---|
by sonication, centrifugation, heating of the soluble fraction, anion-exchange and hydrophobic interaction chromatography | Thermococcus kodakarensis |
Storage Stability | Organism |
---|---|
50 mM TrisHCl buffer, pH 8.0 | Thermococcus kodakarensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-threonine + NAD+ | - |
Thermococcus kodakarensis | (2S)-2-amino-3-oxobutanoate + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 38016, electrospray mass spectrometry and gel filtration | Thermococcus kodakarensis |
Synonyms | Comment | Organism |
---|---|---|
L-threonine dehydrogenase | - |
Thermococcus kodakarensis |
TDH | - |
Thermococcus kodakarensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Thermococcus kodakarensis |