Cloned (Comment) | Organism |
---|---|
orf PH0655, gene TDH, overexpression in Escherichia coli strain BL21(DE3) | Pyrococcus horikoshii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | complete inhibition at 50 mM | Pyrococcus horikoshii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Pyrococcus horikoshii | |
0.01 | - |
NAD+ | pH 7.5, 65°C | Pyrococcus horikoshii | |
0.013 | - |
L-threonine | pH 7.5, 65°C | Pyrococcus horikoshii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 0.54 mol Ca2+ per mol of enzyme subunit | Pyrococcus horikoshii | |
Cu2+ | 0.29 mol Ca2+ per mol of enzyme subunit | Pyrococcus horikoshii | |
Mg2+ | 0.2 mol Ca2+ per mol of enzyme subunit | Pyrococcus horikoshii | |
Zn2+ | required, 1.22 mol Zn2+ per mol of enzyme subunit | Pyrococcus horikoshii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37742 | - |
2 * 36000-38000, recombinant enzyme, SDS-PAGE, 2 * 37742, sequence calculation | Pyrococcus horikoshii |
76000 | - |
recombinant enzyme, gel filtration | Pyrococcus horikoshii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-threonine + NAD+ | Pyrococcus horikoshii | - |
(2S)-2-amino-3-oxobutanoate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus horikoshii | - |
orf PH0655, gene TDH | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain BL21(DE3) by heat treatment at 85°C for 30 min, centrifugation, ion exchange chromatography, and gel filtration | Pyrococcus horikoshii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-threonine + NAD+ | - |
Pyrococcus horikoshii | (2S)-2-amino-3-oxobutanoate + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 36000-38000, recombinant enzyme, SDS-PAGE, 2 * 37742, sequence calculation | Pyrococcus horikoshii |
Synonyms | Comment | Organism |
---|---|---|
L-threonine dehydrogenase | - |
Pyrococcus horikoshii |
TDH | - |
Pyrococcus horikoshii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
assay at | Pyrococcus horikoshii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 106 | - |
Pyrococcus horikoshii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
108 | - |
2-step thermal inactivation | Pyrococcus horikoshii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.2 | - |
NAD+ | pH 7.5, 65°C | Pyrococcus horikoshii | |
7.2 | - |
L-threonine | pH 7.5, 65°C | Pyrococcus horikoshii | |
551.7 | - |
NAD+ | pH 7.5, 65°C | Pyrococcus horikoshii | |
551.7 | - |
L-threonine | pH 7.5, 65°C | Pyrococcus horikoshii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Pyrococcus horikoshii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | dependent on | Pyrococcus horikoshii |