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Literature summary for 1.1.1.103 extracted from

  • Machielsen, R.; van der Oost, J.
    Production and characterization of a thermostable L-threonine dehydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus (2006), FEBS J., 273, 2722-2729.
    View publication on PubMed
Search for more literature for 1.1.1.103

Cloned(Commentary)

Cloned (Comment) Organism
gene tdh, functional expression in Escherichia coli Pyrococcus furiosus

Inhibitors

Inhibitors Comment Organism Structure
EDTA complete inhibition, fully reversible by Zn2+ or Co2+ Pyrococcus furiosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.055
-
 NAD+ pH 7.0, 70°C, recombinant enzyme Pyrococcus furiosus
1.5
-
 L-threonine pH 7.0, 70°C, recombinant enzyme Pyrococcus furiosus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates Pyrococcus furiosus
Zn2+ 2 Zn2+ per enzyme subunit, binding involves residues G168, G175, G180, and G212, overview Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37823
-
 4 * 40000, recombinant enzyme, SDS-PAGE, 4 * 37823, sequence calculation Pyrococcus furiosus
40000
-
 4 * 40000, recombinant enzyme, SDS-PAGE, 4 * 37823, sequence calculation Pyrococcus furiosus
155000
-
 about, recombinant enzyme, gel filtration Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-threonine + NAD+ Pyrococcus furiosus
-
 (2S)-2-amino-3-oxobutanoate + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus
-
 gene tdh
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme 48fold from Escherichia coli to homogeneity by heat treatment and anion exchange chromatography Pyrococcus furiosus

Reaction

Reaction Comment Organism Reaction ID
L-threonine + NAD+ = L-2-amino-3-oxobutanoate + NADH + H+ His94 is an active site residue, substrate binding involved residues Gly66, Gly71, Gly77, and Val80 Pyrococcus furiosus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
butane-2,3-diol + NAD+
-
 Pyrococcus furiosus ? + NADH
-
 ?
D-threonine + NAD+
-
 Pyrococcus furiosus D-2-amino-3-oxobutyrate + NADH
-
 ?
L-serine + NAD+
-
 Pyrococcus furiosus ? + NADH
-
 ?
L-threonine + NAD+
-
 Pyrococcus furiosus (2S)-2-amino-3-oxobutanoate + NADH + H+
-
 ?
L-threonine + NAD+ substrate binding involved residues G66, G71, G77, and V80, H94 is an active site residue Pyrococcus furiosus (2S)-2-amino-3-oxobutanoate + NADH + H+
-
 ?
additional information broad substrate specificity, determinants, overview Pyrococcus furiosus ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 40000, recombinant enzyme, SDS-PAGE, 4 * 37823, sequence calculation Pyrococcus furiosus

Synonyms

Synonyms Comment Organism
L-threonine dehydrogenase
-
 Pyrococcus furiosus
TDH
-
 Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
 assay at Pyrococcus furiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Pyrococcus furiosus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Pyrococcus furiosus