Inhibitors | Comment | Organism | Structure |
---|---|---|---|
divalent cations | i.e. Cu2+, Co2+, Ca2+, Mg2+, Mn2+, Ni2+, Zn2+, strong inhibition | Saccharomyces cerevisiae | |
EDTA | weak inhibition | Saccharomyces cerevisiae | |
F- | weak inhibition | Saccharomyces cerevisiae | |
N-ethylmaleimide | strong inhibitor | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
NADPH | cosubstrate hexadecyldihydroxyacetone phosphate | Saccharomyces cerevisiae | |
0.02 | - |
NADPH | - |
Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyldihydroxyacetone phosphate + NADPH | Saccharomyces cerevisiae | acyldihydroxyacetone phosphate pathway | 1-acyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
acyldihydroxyacetone phosphate + NADPH | Saccharomyces cerevisiae | reaction in non-ether lipids biosynthesis | 1-acyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
alkyldihydroxyacetone phosphate + NADPH + H+ | Saccharomyces cerevisiae | acyldihydroxyacetone phosphate pathway | 1-alkyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
alkyldihydroxyacetone phosphate + NADPH + H+ | Saccharomyces cerevisiae | reaction in ether lipids biosynthesis | 1-alkyl-sn-glycerol 3-phosphate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
wild-type strain | - |
Storage Stability | Organism |
---|---|
-20°C, membrane fraction stable | Saccharomyces cerevisiae |
-80°C, membrane fraction stable | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyldihydroxyacetone phosphate + NADPH | - |
Saccharomyces cerevisiae | 1-acyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
acyldihydroxyacetone phosphate + NADPH | acyldihydroxyacetone phosphate pathway | Saccharomyces cerevisiae | 1-acyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
acyldihydroxyacetone phosphate + NADPH | reaction in non-ether lipids biosynthesis | Saccharomyces cerevisiae | 1-acyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
alkyldihydroxyacetone phosphate + NADPH + H+ | - |
Saccharomyces cerevisiae | 1-alkyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
alkyldihydroxyacetone phosphate + NADPH + H+ | acyldihydroxyacetone phosphate pathway | Saccharomyces cerevisiae | 1-alkyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
alkyldihydroxyacetone phosphate + NADPH + H+ | reaction in ether lipids biosynthesis | Saccharomyces cerevisiae | 1-alkyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
hexadecyldihydroxyacetone phosphate + NADPH | - |
Saccharomyces cerevisiae | 1-hexadecyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
palmitoylglycerone phosphate + NADPH | i.e. palmitoyldihydroxyacetone phosphate | Saccharomyces cerevisiae | 1-palmitoylglycerol 3-phosphate + NADP+ | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Saccharomyces cerevisiae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | 43 | about half-maximal activity at 28°C and 43°C | Saccharomyces cerevisiae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
10 min stable below | Saccharomyces cerevisiae |
45 | - |
t1/2: 10 min | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.7 | 7.2 | broad optimum | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | specific for NADPH | Saccharomyces cerevisiae | |
NADPH | hydrogen from B-side of nicotinamide ring is transferred to reduce keto substrates | Saccharomyces cerevisiae |