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Literature summary for 1.1.1.100 extracted from

  • Shimakata, T.; Stumpf, P.K.
    Purification and characterizations of beta-ketoacyl-[acyl-carrier-protein] reductase, beta-hydroxyacyl-[acylcarrier-protein] dehydrase, and enoyl-[acyl-carrier-protein] reductase from Spinacia oleracea leaves (1982), Arch. Biochem. Biophys., 218, 77-91.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
additional information not: N-ethylmaleimide, arsenite Spinacia oleracea
p-chloromercuribenzoate
-
Spinacia oleracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0037
-
acetoacetyl-[acyl-carrier protein]
-
Spinacia oleracea
0.025
-
NADPH
-
Spinacia oleracea
0.25
-
acetoacetyl-CoA
-
Spinacia oleracea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24200
-
4 * 24200, SDS-PAGE Spinacia oleracea
97000
-
gel filtration Spinacia oleracea

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Spinacia oleracea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Spinacia oleracea

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Spinacia oleracea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0032
-
-
Escherichia coli
0.005
-
-
Spinacia oleracea
7.95
-
-
Spinacia oleracea

Storage Stability

Storage Stability Organism
-20°C, stable for 2 months Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-oxoacyl-[acyl-carrier protein] + NADPH
-
Escherichia coli (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
r
3-oxoacyl-[acyl-carrier protein] + NADPH
-
Spinacia oleracea (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
r
acetoacetyl-CoA + NADPH + H+
-
Escherichia coli D-beta-hydroxybutyryl-CoA + NADP+
-
?
acetoacetyl-CoA + NADPH + H+
-
Spinacia oleracea D-beta-hydroxybutyryl-CoA + NADP+
-
?
acetoacetyl-[acyl-carrier protein] + NADPH
-
Escherichia coli D-beta-hydroxybutyryl-[acyl-carrier protein] + NADP+
-
r
acetoacetyl-[acyl-carrier protein] + NADPH
-
Spinacia oleracea D-beta-hydroxybutyryl-[acyl-carrier protein] + NADP+
-
r
additional information L-isomer completely inactive Escherichia coli ?
-
?
additional information L-isomer completely inactive Spinacia oleracea ?
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 24200, SDS-PAGE Spinacia oleracea

pH Range

pH Minimum pH Maximum Comment Organism
6 8.7 both reaction directions Spinacia oleracea

Cofactor

Cofactor Comment Organism Structure
NADH NADPH is much more effective than NADH Spinacia oleracea
NADP+
-
Escherichia coli
NADP+
-
Spinacia oleracea
NADPH
-
Escherichia coli
NADPH
-
Spinacia oleracea