Cloned (Comment) | Organism |
---|---|
gene dhs-21, DNA and amino acid sequence determination and analysis, the dhs-21 gene is physically mapped to the cosmid R11D1 between the sma-1 and mes-4genes on chromosome V, expression of His6-tagged enzyme. Spatial and temporal expression pattern of the DHS-21-GFP construct in transgenic worms, overview | Caenorhabditis elegans |
Protein Variants | Comment | Organism |
---|---|---|
additional information | library screening and isolation of a dhs-21 deletion mutant | Caenorhabditis elegans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
L-erythrulose | pH 7.0, temperature not specified in the publication | Caenorhabditis elegans | |
0.18 | - |
L-ribulose | pH 7.0, temperature not specified in the publication | Caenorhabditis elegans | |
0.27 | - |
D-erythrose | pH 7.0, temperature not specified in the publication | Caenorhabditis elegans | |
0.66 | - |
L-xylulose | pH 7.0, temperature not specified in the publication | Caenorhabditis elegans | |
0.68 | - |
D-threose | pH 7.0, temperature not specified in the publication | Caenorhabditis elegans | |
2.58 | - |
D-xylulose | pH 7.0, temperature not specified in the publication | Caenorhabditis elegans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plasma membrane | of spermatids | Caenorhabditis elegans | 5886 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-xylulose + NADPH + H+ | Caenorhabditis elegans | - |
xylitol + NADP+ | - |
ir | |
additional information | Caenorhabditis elegans | dicarbonyl/L-xylulose reductase is bifunctional | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caenorhabditis elegans | Q21929 | gene dhs-21 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
gonadal sheath cell | - |
Caenorhabditis elegans | - |
intestine | - |
Caenorhabditis elegans | - |
spermathecal-uterine valve | - |
Caenorhabditis elegans | - |
spermatid | - |
Caenorhabditis elegans | - |
uterine seam cell | - |
Caenorhabditis elegans | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-erythrose + NADPH + H+ | - |
Caenorhabditis elegans | ? + NADP+ | - |
ir | |
D-threose + NADPH + H+ | - |
Caenorhabditis elegans | ? + NADP+ | - |
ir | |
D-xylulose + NADPH + H+ | - |
Caenorhabditis elegans | xylitol + NADP+ | - |
? | |
L-erythrulose + NADPH + H+ | - |
Caenorhabditis elegans | ? + NADP+ | - |
ir | |
L-ribulose + NADPH + H+ | low activity | Caenorhabditis elegans | ? + NADP+ | - |
? | |
L-xylulose + NADPH + H+ | - |
Caenorhabditis elegans | xylitol + NADP+ | - |
ir | |
additional information | substrate specificity, overview | Caenorhabditis elegans | ? | - |
? | |
additional information | dicarbonyl/L-xylulose reductase is bifunctional | Caenorhabditis elegans | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DCXR | - |
Caenorhabditis elegans |
dicarbonyl/L-xylulose reductase | - |
Caenorhabditis elegans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.49 | - |
L-ribulose | pH 7.0, temperature not specified in the publication | Caenorhabditis elegans | |
1.22 | - |
D-xylulose | pH 7.0, temperature not specified in the publication | Caenorhabditis elegans | |
1.8 | - |
D-erythrose | pH 7.0, temperature not specified in the publication | Caenorhabditis elegans | |
2.32 | - |
D-threose | pH 7.0, temperature not specified in the publication | Caenorhabditis elegans | |
3.32 | - |
L-erythrulose | pH 7.0, temperature not specified in the publication | Caenorhabditis elegans | |
4.98 | - |
L-xylulose | pH 7.0, temperature not specified in the publication | Caenorhabditis elegans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Caenorhabditis elegans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with NADH | Caenorhabditis elegans | |
NADPH | - |
Caenorhabditis elegans |
Organism | Comment | Expression |
---|---|---|
Caenorhabditis elegans | knock-down of daf-16 and elt-2 transcription factors affects dhs-21 expression | down |
General Information | Comment | Organism |
---|---|---|
malfunction | dhs-21 null mutants show defects in longevity, reproduction and egg-laying | Caenorhabditis elegans |
metabolism | dicarbonyl/L-xylulose reductase converts L-xylulose into xylitol, and reduces various alpha-dicarbonyl compounds, performing a dual role in carbohydrate metabolism and detoxification | Caenorhabditis elegans |
physiological function | the enzyme is essential for normal life span and reproduction | Caenorhabditis elegans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.36 | - |
L-ribulose | pH 7.0, temperature not specified in the publication | Caenorhabditis elegans | |
0.47 | - |
D-xylulose | pH 7.0, temperature not specified in the publication | Caenorhabditis elegans | |
3.42 | - |
D-threose | pH 7.0, temperature not specified in the publication | Caenorhabditis elegans | |
6.75 | - |
D-erythrose | pH 7.0, temperature not specified in the publication | Caenorhabditis elegans | |
7.55 | - |
L-xylulose | pH 7.0, temperature not specified in the publication | Caenorhabditis elegans | |
19.03 | - |
L-erythrulose | pH 7.0, temperature not specified in the publication | Caenorhabditis elegans |