Literature summary for 1.1.1.10 extracted from

Hou, J.; Shen, Y.; Li, X.P.; Bao, X.M.
Effect of the reversal of coenzyme specificity by expression of mutated Pichia stipitis xylitol dehydrogenase in recombinant Saccharomyces cerevisiae (2007), Lett. Appl. Microbiol., 45, 184-189.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression of Pichia stipitis mutated XYL2 (D207/I208R/F209S or XYL2 S96C/S99C/Y102C/D207A/I208R/F209S) in Saccharomyces cerevisiae	Scheffersomyces stipitis

Protein Variants

Protein Variants	Comment	Organism
D207/I208R/F209S	kcat/Km of mutant enzyme for NAD+ dropps 15fold compared with the native enzyme, kcat/Km for NADP+ increases up to 4100fold	Scheffersomyces stipitis
S96C/S99C/Y102C/D207A/I208R/F209S	mutation produces a further 4fold improvement in the kcat/Km for NADP+ compared to mutant enzyme D207/I208R/F209S	Scheffersomyces stipitis

Organism

Organism	UniProt	Comment	Textmining
Scheffersomyces stipitis	-	-	-

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Release 2023.1 (January 2023)