Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | 10% v/v, 3fold increase in activity | Pyrobaculum calidifontis |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pyrobaculum calidifontis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | presence of EDTA is not significantly inhibitory. Incubation with 100 mM EDTA at 70°C for 2 h results in loss of half of the enzyme activity. The enzyme is highly stable in the presence of up to 8 M urea or up to 6 M guanidine hydrochloride | Pyrobaculum calidifontis | |
Sodium dodecyl sulfate | 5 mM, complete loss of activity | Pyrobaculum calidifontis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.072 | - |
NADH | pH 6, 60°C | Pyrobaculum calidifontis | |
0.36 | - |
1,4-Butanediol | pH 10, 60°C | Pyrobaculum calidifontis | |
0.67 | - |
Glutaraldehyde | pH 6, 60°C | Pyrobaculum calidifontis | |
1.7 | - |
NAD+ | pH 10, 60°C | Pyrobaculum calidifontis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | Pcal_1311 is contains two zinc atoms per subunit. Twofold increase in enzyme activity of Pcal_1311 when produced in the presence of 25 microM Zn2+ as compared to the protein produced in tap water | Pyrobaculum calidifontis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
76000 | - |
gel filtration | Pyrobaculum calidifontis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrobaculum calidifontis | A3MVR8 | - |
- |
Pyrobaculum calidifontis JCM 11548 | A3MVR8 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-butanediol + NAD+ | - |
Pyrobaculum calidifontis | ? + NADH + H+ | - |
? | |
1,4-butanediol + NAD+ | best substrate in oxidation reaction | Pyrobaculum calidifontis | ? + NADH + H+ | - |
? | |
1-butanol + NAD+ | - |
Pyrobaculum calidifontis | butanal + NADH + H+ | - |
? | |
1-butanol + NAD+ | - |
Pyrobaculum calidifontis JCM 11548 | butanal + NADH + H+ | - |
? | |
1-hexanol + NAD+ | - |
Pyrobaculum calidifontis | hexanal + NADH + H+ | - |
? | |
1-hexanol + NAD+ | - |
Pyrobaculum calidifontis JCM 11548 | hexanal + NADH + H+ | - |
? | |
1-octanol + NAD+ | - |
Pyrobaculum calidifontis | octanal + NADH + H+ | - |
? | |
1-octanol + NAD+ | - |
Pyrobaculum calidifontis JCM 11548 | octanal + NADH + H+ | - |
? | |
1-pentanol + NAD+ | - |
Pyrobaculum calidifontis | pentanal + NADH + H+ | - |
? | |
acetaldehyde + NADH + H+ | 5.7% of the activity with glutaraldehyde | Pyrobaculum calidifontis | ethanol + NAD+ | - |
? | |
acetaldehyde + NADH + H+ | 5.7% of the activity with glutaraldehyde | Pyrobaculum calidifontis JCM 11548 | ethanol + NAD+ | - |
? | |
cinnamyl alcohol + NAD+ | - |
Pyrobaculum calidifontis | cinnamyl aldehyde + NADH + H+ | - |
? | |
ethanol + NAD+ | 33% of the activity with 1,4-butanediol | Pyrobaculum calidifontis | acetaldehyde + NADH + H+ | - |
? | |
glutaraldehyde + NADH + H+ | best substrate in reduction reaction | Pyrobaculum calidifontis | ? + NAD+ | - |
? | |
hexanal + NADH + H+ | 69.6% of the activity with glutaraldehyde | Pyrobaculum calidifontis | 1-hexanol + NAD+ | - |
? | |
isobutanal + NADH + H+ | 20% of the activity with glutaraldehyde | Pyrobaculum calidifontis | isobutanol + NAD+ | - |
? | |
mannitol + NAD+ | - |
Pyrobaculum calidifontis | ? + NADH + H+ | - |
? | |
additional information | the enzyme catalyzes NAD(H)-dependent oxidation of various alcohols and reduction of aldehydes, with a marked preference for substrates with functional group at the terminal carbon atom | Pyrobaculum calidifontis | ? | - |
? | |
additional information | the enzyme catalyzes NAD(H)-dependent oxidation of various alcohols and reduction of aldehydes, with a marked preference for substrates with functional group at the terminal carbon atom | Pyrobaculum calidifontis JCM 11548 | ? | - |
? | |
pentanal + NADH + H+ | 38% of the activity with glutaraldehyde | Pyrobaculum calidifontis | 1-pentanol + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 37555, calculated | Pyrobaculum calidifontis |
Synonyms | Comment | Organism |
---|---|---|
alcohol dehydrogenase GroES domain protein | - |
Pyrobaculum calidifontis |
Pcal_1311 | - |
Pyrobaculum calidifontis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Pyrobaculum calidifontis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
12 h, 80% residual activity | Pyrobaculum calidifontis |
90 | - |
3 h, 90% residual activity. No significant change in the CD spectra up to 90 °C | Pyrobaculum calidifontis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.98 | - |
1,4-Butanediol | pH 10, 60°C | Pyrobaculum calidifontis | |
93.13 | - |
Glutaraldehyde | pH 6, 60°C | Pyrobaculum calidifontis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
sodium phosphate buffer, highest activity for reduction of acetaldehyde | Pyrobaculum calidifontis |
10 | - |
glycineNaOH buffer, highest activity for oxidation of ethanol | Pyrobaculum calidifontis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with NADP+ | Pyrobaculum calidifontis | |
NAD+ | - |
Pyrobaculum calidifontis | |
NADH | - |
Pyrobaculum calidifontis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pyrobaculum calidifontis | - |
- |
6.6 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.5 | - |
1,4-Butanediol | pH 10, 60°C | Pyrobaculum calidifontis | |
139 | - |
Glutaraldehyde | pH 6, 60°C | Pyrobaculum calidifontis |