Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetivibrio thermocellus | A3DCI2 | - |
- |
Acetivibrio thermocellus DSM 1237 | A3DCI2 | - |
- |
Thermoanaerobacterium saccharolyticum | I3VSF1 | - |
- |
Thermoanaerobacterium saccharolyticum DSM 8691 | I3VSF1 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.01 | - |
gene AdhE deletion mutant, cosubstrate NADH, pH 7.0, 55°C | Thermoanaerobacterium saccharolyticum |
0.03 | - |
gene AdhE deletion mutant, cosubstrate NADH, pH 7.0, 55°C | Acetivibrio thermocellus |
0.05 | - |
wild-type, cosubstrate NADPH, pH 7.0, 55°C | Acetivibrio thermocellus |
0.07 | - |
gene AdhE deletion mutant, cosubstrate NADPH, pH 7.0, 55°C | Acetivibrio thermocellus |
0.25 | - |
gene AdhE deletion mutant, cosubstrate NADPH, pH 7.0, 55°C | Thermoanaerobacterium saccharolyticum |
0.68 | - |
wild-type, cosubstrate NADPH, pH 7.0, 55°C | Thermoanaerobacterium saccharolyticum |
1.52 | - |
wild-type, cosubstrate NADH, pH 7.0, 55°C | Thermoanaerobacterium saccharolyticum |
7.68 | - |
wild-type, cosubstrate NADH, pH 7.0, 55°C | Acetivibrio thermocellus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + NADH + H+ | - |
Acetivibrio thermocellus | ethanol + NAD+ | - |
r | |
acetaldehyde + NADH + H+ | - |
Thermoanaerobacterium saccharolyticum | ethanol + NAD+ | - |
? | |
acetaldehyde + NADH + H+ | - |
Acetivibrio thermocellus DSM 1237 | ethanol + NAD+ | - |
r | |
acetaldehyde + NADH + H+ | - |
Thermoanaerobacterium saccharolyticum DSM 8691 | ethanol + NAD+ | - |
? | |
acetaldehyde + NADPH + H+ | - |
Acetivibrio thermocellus | ethanol + NADP+ | - |
r | |
acetaldehyde + NADPH + H+ | - |
Thermoanaerobacterium saccharolyticum | ethanol + NADP+ | - |
? | |
acetaldehyde + NADPH + H+ | - |
Acetivibrio thermocellus DSM 1237 | ethanol + NADP+ | - |
r | |
acetaldehyde + NADPH + H+ | - |
Thermoanaerobacterium saccharolyticum DSM 8691 | ethanol + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AdhE | - |
Acetivibrio thermocellus |
AdhE | - |
Thermoanaerobacterium saccharolyticum |
AdhE | bifunctional alcohol and aldehyde dehydrogenase gene | Acetivibrio thermocellus |
AdhE | bifunctional alcohol and aldehyde dehydrogenase gene | Thermoanaerobacterium saccharolyticum |
Tsac_0416 | - |
Thermoanaerobacterium saccharolyticum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Acetivibrio thermocellus | |
NADH | - |
Thermoanaerobacterium saccharolyticum | |
NADPH | - |
Acetivibrio thermocellus | |
NADPH | - |
Thermoanaerobacterium saccharolyticum |
General Information | Comment | Organism |
---|---|---|
physiological function | deletion of the bifunctional alcohol and aldehyde dehydrogenase gene adhE reduces ethanol production by more than 95%. In the deletion strain, fermentation products shift from ethanol to lactate production and result in lower cell density and longer time to reach maximal cell density. It loses more than 85% of alcohol dehydrogenase activity. Aldehyde dehydrogenase activity does not appear to be affected, although its activity is low in cell extracts. Adding ubiquinone-0 to the aldehyde dehydrogenase assay increases activity in the parent strain but does not increase activity in the adhE deletion strain | Thermoanaerobacterium saccharolyticum |
physiological function | deletion of the bifunctional alcohol and aldehyde dehydrogenase gene adhE reduces ethanol production by more than 95%. In the deletion strains, fermentation products shift from ethanol to lactate production and result in lower cell density and longer time to reach maximal cell density. The deletion strain additionally contains a point mutation in the lactate dehydrogenase gene, which appears to deregulate its activation by fructose 1,6-bisphosphate, leading to constitutive activation of lactate dehydrogenase | Acetivibrio thermocellus |