Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibitory: Triton X-100 at 1%, guanidinium hydrochloride at 0.2 M | Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
4 * 37000, SDS-PAGE | Geobacillus stearothermophilus |
158000 | - |
gel filtration | Geobacillus stearothermophilus |
Organic Solvent | Comment | Organism |
---|---|---|
1-butanol | 10%, 29% residual activity | Geobacillus stearothermophilus |
1-propanol | 10%, 38% residual activity | Geobacillus stearothermophilus |
Ethanol | 10%, 86% residual activity | Geobacillus stearothermophilus |
Methanol | 10%, 100% residual activity | Geobacillus stearothermophilus |
urea | 0.1 M, 40% residual activity | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | P42328 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methoxybenzaldehyde + NADH + H+ | - |
Geobacillus stearothermophilus | 4-methoxybenzyl alcohol + NAD+ | 51% of the activity with butan-2-ol | ? | |
acetaldehyde + NADH + H+ | - |
Geobacillus stearothermophilus | ethanol + NAD+ | 100% of the activity with butan-2-ol | r | |
benzaldehyde + NADH + H+ | - |
Geobacillus stearothermophilus | benzyl alcohol + NAD+ | 93% of the activity with butan-2-ol | r | |
benzyl alcohol + NAD+ | - |
Geobacillus stearothermophilus | benzaldehyde + NADH + H+ | 33% of the activity with butan-2-ol | r | |
butan-2-ol + NAD+ | - |
Geobacillus stearothermophilus | butanone + NADH + H+ | 83% of the activity with butan-2-ol | ? | |
cyclopentanol + NAD+ | - |
Geobacillus stearothermophilus | cyclopentanone + NADH + H+ | 92% of the activity with butan-2-ol | ? | |
ethanol + NAD+ | - |
Geobacillus stearothermophilus | acetaldehyde + NADH + H+ | 83% of the activity with butan-2-ol | r | |
pentan-1-ol + NAD+ | - |
Geobacillus stearothermophilus | pentanal + NADH + H+ | 76% of the activity with butan-2-ol | ? | |
pentan-2-ol + NAD+ | - |
Geobacillus stearothermophilus | pentan-2-one + NADH + H+ | 93% of the activity with butan-2-ol | ? | |
prop-2-en-1-ol + NAD+ | - |
Geobacillus stearothermophilus | prop-2-enal + NADH + H+ | 85% of the activity with butan-2-ol | ? | |
propan-1-ol + NAD+ | - |
Geobacillus stearothermophilus | propanal + NADH + H+ | 68% of the activity with butan-2-ol | r | |
propan-2-ol + NAD+ | - |
Geobacillus stearothermophilus | acetone + NADH + H+ | 79% of the activity with butan-2-ol | ? | |
propanal + NADH + H+ | - |
Geobacillus stearothermophilus | propan-1-ol + NAD+ | 38% of the activity with butan-2-ol | r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 37000, SDS-PAGE | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
reduction of acetaldehyde | Geobacillus stearothermophilus |
8 | - |
oxidation of ethanol | Geobacillus stearothermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Geobacillus stearothermophilus | |
NADH | - |
Geobacillus stearothermophilus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Geobacillus stearothermophilus | isoelectric focusing | - |
4.9 |