Cloned (Comment) | Organism |
---|---|
homologously expressed | Haloferax volcanii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.071 | - |
NADH | pH 6.0, temperature not specified in the publication | Haloferax volcanii | |
0.29 | - |
NAD+ | pH 11.0, temperature not specified in the publication | Haloferax volcanii | |
28 | - |
acetaldehyde | pH 6.0, temperature not specified in the publication | Haloferax volcanii | |
31 | - |
ethanol | pH 11.0, temperature not specified in the publication | Haloferax volcanii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | optimally active with ethanol and 1-propanol at pH 11.0 with 3 M KCl and with 1-butanol at pH 10.0 with 4 M KCl. Catalyzes the reductive reaction optimally at pH 6.0 with 4 M KCl | Haloferax volcanii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37600 | - |
4 * 37600, SDS-PAGE | Haloferax volcanii |
138000 | - |
gel filtration | Haloferax volcanii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haloferax volcanii | D4GSN2 | - |
- |
Haloferax volcanii DSM 3757 | D4GSN2 | - |
- |
Purification (Comment) | Organism |
---|---|
purified in one step by immobilized metal-affinity chromatography | Haloferax volcanii |
Storage Stability | Organism |
---|---|
-20°C, inactive after approximately 2 weeks, storage at -80°C or lyophilization does not improve storage time | Haloferax volcanii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-propanol + NAD+ | the enzyme shows a preference for short-chain alcohols ethanol and 1-propanol | Haloferax volcanii | propanal + NADH + H+ | - |
? | |
1-propanol + NAD+ | the enzyme shows a preference for short-chain alcohols ethanol and 1-propanol | Haloferax volcanii DSM 3757 | propanal + NADH + H+ | - |
? | |
3-methylbutanal + NAD+ | about 10% of the activity with ethanol or 1-propanol | Haloferax volcanii | ? + NADH + H+ | - |
? | |
3-methylbutanal + NAD+ | about 10% of the activity with ethanol or 1-propanol | Haloferax volcanii DSM 3757 | ? + NADH + H+ | - |
? | |
butanol + NAD+ | about 15% of the activity with ethanol or 1-propanol | Haloferax volcanii | 1-butanal + NADH + H+ | - |
? | |
ethanol + NAD+ | the enzyme shows a preference for short-chain alcohols ethanol and 1-propanol | Haloferax volcanii | acetaldehyde + NADH + H+ | - |
? | |
ethanol + NAD+ | the enzyme shows a preference for short-chain alcohols ethanol and 1-propanol | Haloferax volcanii DSM 3757 | acetaldehyde + NADH + H+ | - |
? | |
additional information | the NAD+-dependent HvADH1 shows a preference for short-chain alcohols, no activity with methanol | Haloferax volcanii | ? | - |
? | |
additional information | the NAD+-dependent HvADH1 shows a preference for short-chain alcohols, no activity with methanol | Haloferax volcanii DSM 3757 | ? | - |
? | |
pentanol + NAD+ | about 10% of the activity with ethanol or 1-propanol | Haloferax volcanii | pentanal + NADH + H+ | - |
? | |
pentanol + NAD+ | about 10% of the activity with ethanol or 1-propanol | Haloferax volcanii DSM 3757 | pentanal + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 37600, SDS-PAGE | Haloferax volcanii |
Synonyms | Comment | Organism |
---|---|---|
HvADH1 | - |
Haloferax volcanii |
HVO_2428 | locus name | Haloferax volcanii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Haloferax volcanii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 90 | 60°C: 75% of maximal activity, 90°C: 75% of maximal activity | Haloferax volcanii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
reduction of aldehyde | Haloferax volcanii |
10 | - |
optimally active with 1-butanol at pH 10.0 with 4 M KCl | Haloferax volcanii |
11 | - |
optimally active with ethanol and 1-propanol at pH 11.0 with 3 M KCl | Haloferax volcanii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | exclusively NAD+ dependent | Haloferax volcanii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.048 | - |
acetaldehyde | pH 6.0, temperature not specified in the publication | Haloferax volcanii | |
0.072 | - |
ethanol | pH 11.0, temperature not specified in the publication | Haloferax volcanii | |
9.037 | - |
NAD+ | pH 11.0, temperature not specified in the publication | Haloferax volcanii | |
32.77 | - |
NADH | pH 6.0, temperature not specified in the publication | Haloferax volcanii |